ACQUISITION OF AN ULTRA HIGH FIELD NMR SPECTROMETER

杜克大学将购买750或800 MHz核磁共振、核磁共振、光谱仪，用于基础生化和生物医学研究的前沿调查。该光谱仪的使用将与现有低场仪器的调度和使用相配合，以便其超高场以及更高的灵敏度和分辨率可以致力于表征重要生物大分子的结构/功能和动力学方面最具挑战性的研究问题。杜克大学生物化学和核磁共振中心的六名基础科学家，以及来自北卡罗来纳大学和北卡罗来纳州立大学的三名研究人员组成了最初的主要用户。该光谱仪还将供其他有这种最先进能力的人使用，以解决不同寻常的兴趣和重要性的项目。在以下领域的研究将取得重大进展：DNA调节和修复蛋白质和组装体RNA-蛋白质催化复合物酶在合成膜脂类、膜相关配体、修饰RNA细胞信号酶和多肽结合转运蛋白蛋白质构象动力学研究大型蛋白质和蛋白质复合体结构的方法论拟议的超高场光谱仪是杜克大学仪器能力的重大新补充，将成为该地区真正独特的资源。它将位于杜克磁共振波谱中心，并作为该中心的一部分发挥作用，该共享仪器设施将极大地加强该机构、研究三角地区和该地区目前强大的生物医学项目的研究工作。

Duke University will purchase a 750 or 800 MHz nuclear magnetic resonance, NMR, spectrometer for frontier investigation of basic biochemical and biomedical research. Use of this spectrometer will be coordinated with scheduling and use of existing lower field instruments so that its ultra high field and enhanced sensitivity and resolution an be dedicated to the most challenging research problems in characterization of structure/function and dynamics of important biological macromolecules. Six basic scientists in Biochemistry and the NMR Center at Duke, along with three investigators from the University of North Carolina and North Carolina State University, comprise the initial major users. The spectrometer will also be available for use by others who have a demonstrated need for this state-of-the-art capability to address projects of unusual interest and importance. Research in the following areas will be significant advanced: DNA regulation and repair proteins and assemblies RNA-protein catalytic complexes Enzymes important in the synthesis of membrane lipids Membrane associated ligands Modified RNAs Cell signaling enzymes and peptide binding transporter proteins Protein conformational dynamics Methodology for structural studies of large proteins and protein complexes The proposed ultra highfield spectrometer represents a significant new addition to the instrumentation capabilities at Duke and will serve as a truly unique resource in the region. It will be located in and function as part of the Duke Magnetic Resonance Spectroscopy Center, a shared instrument facility will substantially enhance research efforts in currently strong biomedical programs at this institution, in the Research Triangle, and in the region.