ACQUISITION OF AN ULTRA HIGH FIELD NMR SPECTROMETER

获取超高场核磁共振波谱仪

• 批准号: 2803018
• 负责人: LEONARD D SPICER
• 金额: $ 40万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2803018
• 关键词: bioimaging /biomedical imaging; biomedical equipment; biomedical equipment purchase; nuclear magnetic resonance spectroscopy; structural biology

Duke University will purchase a 750 or 800 MHz nuclear magnetic resonance, NMR, spectrometer for frontier investigation of basic biochemical and biomedical research. Use of this spectrometer will be coordinated with scheduling and use of existing lower field instruments so that its ultra high field and enhanced sensitivity and resolution an be dedicated to the most challenging research problems in characterization of structure/function and dynamics of important biological macromolecules. Six basic scientists in Biochemistry and the NMR Center at Duke, along with three investigators from the University of North Carolina and North Carolina State University, comprise the initial major users. The spectrometer will also be available for use by others who have a demonstrated need for this state-of-the-art capability to address projects of unusual interest and importance. Research in the following areas will be significant advanced: DNA regulation and repair proteins and assemblies RNA-protein catalytic complexes Enzymes important in the synthesis of membrane lipids Membrane associated ligands Modified RNAs Cell signaling enzymes and peptide binding transporter proteins Protein conformational dynamics Methodology for structural studies of large proteins and protein complexes The proposed ultra highfield spectrometer represents a significant new addition to the instrumentation capabilities at Duke and will serve as a truly unique resource in the region. It will be located in and function as part of the Duke Magnetic Resonance Spectroscopy Center, a shared instrument facility will substantially enhance research efforts in currently strong biomedical programs at this institution, in the Research Triangle, and in the region.

杜克大学将购买一台750或800兆赫的核磁共振、核磁共振、光谱仪，用于基础生化和生物医学研究的前沿研究。该光谱仪的使用将与现有的低场仪器的调度和使用相协调，以便其超高场和增强的灵敏度和分辨率可以致力于重要生物大分子结构/功能和动力学表征中最具挑战性的研究问题。杜克大学生物化学和核磁共振中心的六位基础科学家，以及北卡罗来纳大学和北卡罗莱纳州立大学的三位研究人员，组成了最初的主要用户。该光谱仪也将提供给其他已经证明需要这种最先进的能力来解决不寻常的兴趣和重要性项目的人使用。以下领域的研究将取得重大进展：DNA调节和修复蛋白和组装rna -蛋白质催化复合物在膜脂合成中的重要酶膜相关配体修饰rna细胞信号转导酶和肽结合转运蛋白蛋白质构象动力学大蛋白质和蛋白质复合物结构研究的方法提出的超高场光谱仪代表了杜克大学仪器能力的重要新补充，并将作为一个真正的该地区独一无二的资源。它将位于并作为杜克磁共振光谱中心的一部分，一个共享的仪器设施将大大加强该机构、研究三角和该地区目前强大的生物医学项目的研究工作。