Replacement Equipment Components for an 800 MHz NMR Spectrometer

800 MHz NMR 波谱仪的替换设备组件

• 批准号: 7796474
• 负责人: LEONARD D SPICER
• 金额: $ 44.17万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-20 至 2011-08-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7796474
• 关键词: Antibodies; Area; Biochemical; Cells; Data; Enzymes; Equipment; Failure; Funding; Generations; Government; HIV vaccine; Housing; Investments; Laboratories; Lipids; Maintenance; Membrane; Monitor; NMR Spectroscopy; Peptides; Phospholipids; Protein NMR Spectroscopy; Proteins; Pump; Research; Research Activity; Research Personnel; Research Project Grants; Resources; Schedule; Science; Scientist; Signal Transduction; System; Time; Universities; Update; Vaccine Design; aged; cost; cost effective; design; frontier; immunogenic; instrument; method development; repaired

DESCRIPTION (provided by applicant): Duke University proposes to replace, update, and enhance ts 800 MHz NMR spectroscopy system with a new console and pumped magnet monitoring data logger. The 800 MHz spectrometer is housed in and managed by the Duke NMR Spectroscopy Center which serves as an NMR resource for all university research projects at Duke and for scientists at universities and government laboratories in the Research Triangle and the Southeastern region. It supports frontier research in basic biochemical and biomedical sciences. The requested new equipment components are needed to replace original equipment installed with the system over 10 years ago, that is now failing at an unacceptable rate. This has significantly impacted research activities of numerous investigators who depend on the Duke 800 instrument to pursue their funded projects and who were already using it in the range of 90-95% of a 24/7 schedule, year around within 4 years of installation. Currently, however, the available time has been more limited due to unanticipated maintenance and repairs associated with failure of critical components as they have aged. This limitation in the instrument time available for research is compounded by the fact that the Varian Inova console and magnet monitoring system are no longer manufactured, having been replaced by a new generation spectrometer and cryogen monitoring system. New parts for our system in some cases are no longer available, and refurbished, used parts are often not immediately available, either. The new equipment components requested for our 800 MHz NMR system provide a cost effective strategy to restore this heavily used and unique spectrometer in this region to its designed capability at a fraction of the cost of a new system. In addition, replacement of the magnet monitoring system protects the investment in our pumped 18.9 Tesla NMR magnet valued at greater than $1M. Current research activities in the following areas will be enhanced by this upgrade to the Duke 800: studies of protein assemblies; protein conformational dynamics and folding; membrane associated lipids and enzymes involved in their synthesis; enzyme structural characterization and redesign; in-cell NMR spectroscopy of proteins; antibody recognition of immunogenic peptides and phospholipids associated with HIV vaccine design; cellular signaling; and methods development for rapid acquisition of multidimensional NMR data.

描述(由申请人提供)：杜克大学提议用一个新的控制台和泵浦磁铁监测数据记录器来取代、更新和增强TS 800 MHz核磁共振波谱系统。800 MHz光谱仪安装在杜克大学核磁共振波谱中心内并由其管理，该中心为杜克大学的所有研究项目以及研究三角地区和东南部地区的大学和政府实验室的科学家提供核磁共振资源。它支持基础生化和生物医学科学的前沿研究。需要所需的新设备部件，以替换10多年前随系统一起安装的原始设备，而这些设备现在以不可接受的速度出现故障。这严重影响了许多研究人员的研究活动，他们依赖Duke 800仪器来进行他们资助的项目，并且在安装后4年内，他们已经在24/7时间表的90%-95%的范围内使用它。然而，目前可用时间更加有限，因为随着关键部件的老化，与故障相关的意外维护和维修。瓦里安Inova控制台和磁体监测系统不再生产，取而代之的是新一代光谱仪和低温监测系统，这加剧了可用于研究的仪器时间的限制。在某些情况下，我们系统的新部件不再可用，翻新过的旧部件通常也不能立即获得。我们的800 MHz核磁共振系统所需的新设备组件提供了一种经济高效的策略，可以将该地区使用频繁且独一无二的光谱仪恢复到其设计能力，而成本仅为新系统的零头。此外，磁铁监控系统的更换保护了我们抽运的18.9特斯拉核磁共振磁铁的投资，价值超过100万美元。通过对Duke 800的升级，以下领域的当前研究活动将得到加强：蛋白质组装研究；蛋白质构象动力学和折叠；参与合成的膜相关脂类和酶；酶结构表征和重新设计；蛋白质的细胞内核磁共振波谱；与艾滋病毒疫苗设计相关的免疫原肽和磷脂的抗体识别；细胞信号；以及快速获取多维核磁共振数据的方法开发。