DNA SEQUENCING SYSTEMS FOR CORE LABORATORY

核心实验室 DNA 测序系统

• 批准号: 2803519
• 负责人: KATHRYN IVANETICH
• 金额: $ 16.47万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2001-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2803519
• 关键词: biomedical equipment; biomedical equipment purchase; computer assisted sequence analysis; nucleic acid quantitation /detection; nucleic acid sequence

The Health Science campus of the University of California at San Francisco is one of the foremost centers of Biomedical research in the world. Its Schools of Medicine, Pharmacy, Dentistry and Nursing employ several hundred outstanding investigators who have an exceptional record in achieving NIH awards. The UCSF campus is served by a state-of-the-art biotechnology core facility, the Biomolecular Resource Center (BRC). The BRC has served the UCSF campus since 1985, and fulfills investigators needs in DNA and peptide sequencing and synthesis. It is housed in 1,700 square feet of renovated laboratory office space. Over 225 principal investigators have used the facility in the last year. Over 190 PIs have had approximately 13,000 DNA templates sequenced. In other services, approximately 140 PIs have had 3,500 oligonucleotides synthesized, approximately 30 PIs have had 115 peptides synthesized, and 25 PIs have had 90 proteins sequenced. The facility is overseen by a Board of Overseers; Dr. Daniel Santi is the Facility Director. Dr. Kathryn Ivanetich, the Associate Director oversees the day-to-day scientific, financial and administrative aspects of the BRC. Funding is requested for an Applied Biosystems 377XL sequencers and Tecan SpectraFluor fluorimetric plate reader. The DNA sequencer is needed to replace an 8 year old sequencer (originally purchased as an ABI 370A) that has become extremely reliable. The fluorimetric plate reader is needed to automate template quantification, which strikingly improves sequencing success rates. The requested instrumentation would provide for more rapid delivery of higher quality DNA sequencing results at lower cost to campus investigators. This request is supported by 25 UCSF Principal Investigators with considerable, ongoing PHS/NIH grant support. The PIs represent 11 departments at UCSF, i.e., Anatomy, Biochemistry and Biophysics, Medicine, Microbiology, Neurosurgery, Pathology, Pharmaceutical Chemistry, Pharmacology, Physiology, Stomatology and Surgery, plus the Cancer Research Institute, the Cardiovascular Research Institute and the Gladstone Institute of Virology and Immunology. The requested instruments would be truly shared resources. No single investigator, group or investigators or department would dominate the use of these instruments.

加州大学旧金山分校的健康科学校区是世界上最重要的生物医学研究中心之一。它的医学院、药房、牙科和护理学院雇用了数百名优秀的研究人员，他们在获得NIH奖项方面有着非凡的记录。加州大学旧金山分校拥有最先进的生物技术核心设施--生物分子资源中心(BRC)。BRC自1985年以来一直服务于加州大学旧金山分校，满足研究人员在DNA和多肽测序和合成方面的需求。它位于翻新后的1700平方英尺的实验室办公空间中。去年，超过225名首席调查人员使用了该设施。超过190个PI已经对大约13,000个DNA模板进行了测序。在其他服务中，大约140个PI合成了3,500个寡核苷酸，大约30个PI合成了115个肽，25个PI合成了90个蛋白质序列。该设施由一个监督委员会监督；丹尼尔·桑蒂博士是设施主任。副主任Kathryn Ivanetich博士负责监督BRC的日常科学、财务和行政方面的工作。申请资金用于应用生物系统377XL测序仪和Tecan spectraFluor荧光平板阅读器。DNA测序仪需要取代已有8年历史的测序仪(最初是作为ABI 370A购买的)，后者已经变得非常可靠。需要荧光平板阅读器来自动进行模板定量，这显著提高了测序成功率。所要求的仪器将提供更快地提供更高质量的DNA测序结果，而校园调查人员的成本更低。这一请求得到了加州大学旧金山分校25名首席调查人员的支持，并得到了PHS/NIH相当大的持续拨款支持。PIs代表加州大学旧金山分校的11个部门，即解剖学、生物化学和生物物理学、医学、微生物学、神经外科、病理学、药物化学、药理学、生理学、口腔和外科，以及癌症研究所、心血管研究所和格拉斯顿病毒学和免疫学研究所。所要求的工具将是真正共享的资源。任何一个调查员、小组或调查员或部门都不会主宰这些工具的使用。

项目成果

Automated template quantification for DNA sequencing facilities.

DNA 测序设施的自动模板定量。

• 发表时间: 2005
• 期刊: Journal of biomolecular techniques : JBT
• 作者: Ivanetich,KathrynM;Yan,Wilson;Wunderlich,KathleenM;Weston,Jennifer;Walkup4th,WardG;Simeon,Christian
• 通讯作者: Simeon,Christian