Quantitative Real Time PCR Instrumentation

加州大学旧金山分校弗朗西斯科的健康科学校区是世界上最重要的生物医学研究中心之一。它的医学，药学，牙科和护理学院雇用了数百名杰出的研究人员，他们在获得NIH奖项方面有着卓越的记录。UCSF校园由最先进的生物技术核心设施-生物分子资源中心（BRC）提供服务。BRC自1985年以来一直为加州大学旧金山分校校园提供服务，满足研究人员在DNA和肽测序、合成、分析和纯化方面的需求。它被安置在1，900平方米。英尺实验室/办公室去年，大约有225名主要调查人员使用了该设施。大约有190个PI有25，500个DNA模板测序，105个PI有大约25，500个DNA模板测序。合成了3,000个寡核苷酸，超过30个PI合成了110个肽，大约20个PI测序了大约79个蛋白质。该设施由监督委员会监督。Kathryn Ivanetich博士担任主任，负责监督布拉迪斯拉发区域中心的日常科学、财务和行政工作。申请资金用于配备Zymark Twister自动化附件的Applied Biosystems 7900 TaqMan定量仪器。该仪器被要求能够提供定量真实的时间PCR作为一个新的BRC服务UCSF的调查，并满足相当大的校园需求，这项技术。这一要求得到了14名UCSF首席研究员的支持。这些PI代表了14个UCSF部门，包括生物化学和生物物理学，医学，解剖学，微生物学和免疫学，内分泌学，神经外科，儿科和口腔科，以及心血管研究所。所请求的插装将是真正的共享资源。没有任何一个调查员、调查员小组或部门会支配其使用。

The Health Sciences campus of the University of California at San Francisco is one of the foremost centers of biomedical research in the world. Its Schools of Medicine, Pharmacy, Dentistry and Nursing employ several hundred outstanding investigators who have an exceptional record in achieving NIH awards. The UCSF campus is served by a state-of-the-art biotechnology core facility, the Biomolecular Resource Center (BRC). The BRC has served the UCSF campus since 1985, and fulfills investigators needs in DNA and peptide sequencing, synthesis, analysis and purification. It is housed in 1,900 sq. ft. of laboratory/office space. Approximately 225 principal investigators have used the facility in the last year. Approximately 190 PIs have had 25,500 DNA templates sequenced, 105 PIs had ca. 3,000 oligonucleotides synthesized, over 30 PIs have had 110 peptides synthesized, and approximately 20 PIs had approximately 79 proteins sequenced. The facility is overseen by a Board of Overseers. Dr. Kathryn Ivanetich is the Director and oversees the day-to-day scientific, financial and administrative aspects of the BRC. Funding is requested for an Applied Biosystems 7900 TaqMan quantitative instrument with a Zymark Twister automation accessory. The instrument is requested to enable the provision of quantitative real time PCR as a new BRC service to UCSF investigators, and to meet the considerable on campus demand for this technology. This request is supported by 14 UCSF Principal Investigators. These PIs represent 14UCSF departments, including Biochemistry and Biophysics, Medicine, Anatomy, Microbiology and Immunology, Endocrinology, Neurological Surgery, Pediatrics, and Stomatology, plus the Cardiovascular Research Institute. The requested instrumentation would be a truly shared resource. No single investigator, group of investigators or department would dominate its use.