Instrumentation for Peptide Synthesis Facility

描述(申请人提供)：加州大学旧金山分校的健康科学校区是世界上最重要的生物医学研究中心之一。它的医学院、药房、牙科和护理学院雇用了数百名优秀的研究人员，他们在获得NIH奖项方面有着非凡的记录。加州大学旧金山分校拥有最先进的生物技术核心设施--生物分子资源中心(BRC)。BRC自1985年以来一直服务于加州大学旧金山分校，满足研究人员在DNA和多肽测序、合成、分析和纯化方面的需求。它的面积为1900平方米。英国《金融时报》实验室/办公室的空间。在过去的一年里，大约有225名首席调查人员使用了该设施。大约190个PI已经对31,150个DNA模板进行了测序，105个PI合成了大约3,100个寡核苷酸，超过30个PI已经合成了155个肽，并且大约20个PI已经对大约50个蛋白质进行了测序。该设施由一个监督委员会监督。Kathryn Ivanetich博士是BRC的主任，负责监督BRC的日常科学、财务和行政方面的工作。申请资金用于应用生物系统433A多肽合成器、Rainin SYMPHONY Multiplex多肽合成器、岛津分析和制备型高效液相系统。这些仪器被要求取代过时和过时的仪器，并提供更高的自动化，为加州大学旧金山分校的研究人员提供快速、廉价和可靠的多肽合成、分析和纯化。这一请求得到了加州大学旧金山分校11名首席调查人员的支持，并获得了相当大的持续的PHS/NIH赠款支持。这些PI代表了加州大学旧金山分校的8个系，包括生物化学和生物物理学、医学、解剖学、微生物学和免疫学、生理学、麻醉学和实验室医学，以及心血管研究所。所请求的工具将是一个真正的共享资源。任何一名调查员、调查组或部门都不会主宰它的使用。

DESCRIPTION (provided by applicant): The Health Sciences campus of the University of California at San Francisco is one of the foremost centers of biomedical research in the world. Its Schools of Medicine, Pharmacy, Dentistry and Nursing employ several hundred outstanding investigators who have an exceptional record in achieving NIH awards. The UCSF campus is served by a state-of-the-art biotechnology core facility, the Biomolecular Resource Center (BRC). The BRC has served the UCSF campus since 1985, and fulfills investigators' needs in DNA and peptide sequencing, synthesis, analysis and purification. It is housed in 1,900 sq. ft. of laboratory/office space. Approximately 225 principal investigators have used the facility in the last year. Approximately 190 PIs have had 31,150 DNA templates sequenced, 105 PIs had ca. 3,100 oligonucleotides synthesized, over 30 PIs have had 155 peptides synthesized, and approximately 20 PIs had approximately 50 proteins sequenced. The facility is overseen by a Board of Overseers. Dr. Kathryn Ivanetich is the Director and oversees the day-to-day scientific, financial and administrative aspects of the BRC. Funding is requested for an Applied Biosystems 433A peptide synthesizer, Rainin Symphony Multiplex multiple peptide synthesizer, a Shimadzu analytical and preparative HPLC systems. The instruments are requested to replace outdated and obsolete instrumentation, and to provide increased automation to provide peptide synthesis, analysis and purification quickly, inexpensively and reliably to UCSF investigators. This request is supported by eleven UCSF Principal Investigators with considerable, ongoing PHS/NIH grant support. These PIs represent 8 UCSF departments, including Biochemistry and Biophysics, Medicine, Anatomy, Microbiology and Immunology, Physiology, Anesthesia, and Laboratory Medicine, plus the Cardiovascular Research Institute. The requested instrumentation would be a truly shared resource. No single investigator, group of investigators or department would dominate its use.