Instrumentation for Peptide Synthesis Facility

描述（由申请人提供）：健康科学校园的加州大学在旧金山弗朗西斯科是一个最重要的中心生物医学研究在世界上。它的医学，药学，牙科和护理学院雇用了数百名杰出的研究人员，他们在获得NIH奖项方面有着卓越的记录。UCSF校园由最先进的生物技术核心设施-生物分子资源中心（BRC）提供服务。BRC自1985年以来一直服务于UCSF校园，并满足研究人员在DNA和肽测序，合成，分析和纯化方面的需求。它被安置在1，900平方米。英尺实验室/办公室去年，大约有225名主要调查人员使用了该设施。大约有190个PI有31，150个DNA模板测序，105个PI有大约31，150个DNA模板测序。合成了3,100种寡核苷酸，超过30个PI合成了155种肽，大约20个PI测序了大约50种蛋白质。该设施由监督委员会监督。Kathryn Ivanetich博士担任主任，负责监督布拉迪斯拉发区域中心的日常科学、财务和行政工作。申请资金用于应用生物系统433 A肽合成仪、Rainin Symphony Multiplex多肽合成仪、Shimadzu分析和制备HPLC系统。这些仪器被要求取代过时和过时的仪器，并提供更高的自动化，以快速，廉价和可靠地为UCSF研究人员提供肽合成，分析和纯化。这一请求得到了11名UCSF首席研究员的支持，并得到了相当大的、持续的PHS/NIH赠款支持。这些PI代表8个UCSF部门，包括生物化学和生物物理学，医学，解剖学，微生物学和免疫学，生理学，麻醉学和实验室医学，加上心血管研究所。所请求的插装将是真正的共享资源。没有任何一个调查员、调查员小组或部门会支配其使用。

DESCRIPTION (provided by applicant): The Health Sciences campus of the University of California at San Francisco is one of the foremost centers of biomedical research in the world. Its Schools of Medicine, Pharmacy, Dentistry and Nursing employ several hundred outstanding investigators who have an exceptional record in achieving NIH awards. The UCSF campus is served by a state-of-the-art biotechnology core facility, the Biomolecular Resource Center (BRC). The BRC has served the UCSF campus since 1985, and fulfills investigators' needs in DNA and peptide sequencing, synthesis, analysis and purification. It is housed in 1,900 sq. ft. of laboratory/office space. Approximately 225 principal investigators have used the facility in the last year. Approximately 190 PIs have had 31,150 DNA templates sequenced, 105 PIs had ca. 3,100 oligonucleotides synthesized, over 30 PIs have had 155 peptides synthesized, and approximately 20 PIs had approximately 50 proteins sequenced. The facility is overseen by a Board of Overseers. Dr. Kathryn Ivanetich is the Director and oversees the day-to-day scientific, financial and administrative aspects of the BRC. Funding is requested for an Applied Biosystems 433A peptide synthesizer, Rainin Symphony Multiplex multiple peptide synthesizer, a Shimadzu analytical and preparative HPLC systems. The instruments are requested to replace outdated and obsolete instrumentation, and to provide increased automation to provide peptide synthesis, analysis and purification quickly, inexpensively and reliably to UCSF investigators. This request is supported by eleven UCSF Principal Investigators with considerable, ongoing PHS/NIH grant support. These PIs represent 8 UCSF departments, including Biochemistry and Biophysics, Medicine, Anatomy, Microbiology and Immunology, Physiology, Anesthesia, and Laboratory Medicine, plus the Cardiovascular Research Institute. The requested instrumentation would be a truly shared resource. No single investigator, group of investigators or department would dominate its use.