NUCLEIC ACID SEQUENCE ANALYSIS

核酸序列分析

• 批准号: 6292216
• 负责人: KATHRYN IVANETICH
• 金额: $ 26.04万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6292216
• 关键词: biomedical equipment purchase; nucleic acid sequence; polymerase chain reaction

DESCRIPTION: (Investigator's Abstract): The Health Sciences campus of the University of California at San Francisco is one of the foremost centers of Biomedical research in the world. Its Schools of Medicine, Pharmacy, Dentistry and Nursing employ several hundred outstanding investigators who have exceptional records in achieving NIH awards. The UCSF campus is served by a state-of-the-art biotechnology core facility, the Biomolecular Resource Center (BRC). The BRC has served the UCSF campus since 1985, and fulfills investigators' needs in DNA and peptide sequencing & synthesis. It is housed in 1,900 sq. ft. of laboratory/office space. Over 235 principal investigators have used the facility over the last year. Over 195 PIs have had ca. 19,000 DNA templates sequenced, approximately 145 PIs have had over 4,000 oligonucteotides synthesized, 30 PIs have had 115 peptides synthesized, and 25 PIs have had 80 proteins sequenced. A Board of Overseers, headed by Dr. Daniel Santi, oversees the facility; Dr. Kathryn Ivanetich is the Associate Director and oversees the day-to-day scientific, financial and administrative aspects of the BRC. Funding is requested for an Applied Biosystems 3700 sequencer (partial funding), a Beckman Biomek 2000 robot and an ABI 7700 TaqMan quantitative PCR instrument. The DNA sequencer is needed to provide expanded automation and capacity to cope with increased demand for DNA sequencing. The robot is needed to automate template quantification/normalization and template preparation, which strikingly improve sequencing success rates. The requested sequencer and robot would provide more rapid delivery of higher quality DNA sequencing results at lower cost to campus investigators. The quantitative PCR is requested to enable the provision of this as a new service to UCSF investigators, and to meet the considerable demand. This request is supported by 24 UCSF Principal Investigators with considerable, ongoing PHS/NIH grant support. The PIs represent 19 UCSF departments. The requested instruments would be truly shared resources. No single investigator, group of investigators or department would dominate their use.

描述：(研究人员摘要)：加州大学旧金山分校的健康科学校区是世界上最重要的生物医学研究中心之一。它的医学院、药房、牙科和护理学院雇用了数百名优秀的研究人员，他们在获得NIH奖项方面有着非凡的记录。加州大学旧金山分校拥有最先进的生物技术核心设施--生物分子资源中心(BRC)。BRC自1985年以来一直服务于加州大学旧金山分校，满足研究人员在DNA和多肽测序与合成方面的需求。它的面积为1900平方米。英国《金融时报》实验室/办公室的空间。在过去的一年里，超过235名主要调查人员使用了该设施。超过195个PI已经对大约19,000个DNA模板进行了测序，大约145个PI已经合成了超过4,000个寡核苷酸，30个PI已经合成了115个肽，并且25个PI已经对80个蛋白质进行了测序。由Daniel Santi博士领导的监督委员会负责监督该设施；Kathryn Ivanetich博士是副主任，负责监督BRC的日常科学、财务和行政方面。申请资金用于一个应用生物系统3700测序仪(部分资金)、一个Beckman Biomek 2000机器人和一个ABI 7700 TaqMan定量聚合酶链式反应仪器。DNA测序仪需要提供扩展的自动化和容量，以应对不断增长的DNA测序需求。需要机器人来自动化模板量化/归一化和模板准备，这显著提高了测序成功率。所要求的测序仪和机器人将以较低的成本向校园调查人员提供更快地提供更高质量的DNA测序结果。要求进行定量聚合酶链式反应，以便能够向加州大学旧金山分校的调查人员提供这项新服务，并满足相当大的需求。这一请求得到了加州大学旧金山分校24名首席调查人员的支持，并获得了相当大的持续的PHS/NIH赠款支持。这些PI代表加州大学旧金山分校的19个部门。所要求的工具将是真正共享的资源。任何一个调查员、调查组或部门都不会主宰它们的使用。