SMALL ANIMAL ECHOCARDIOGRAPHY SYSTEM

小动物超声心动图系统

• 批准号: 2803525
• 负责人: John V. Weil
• 金额: $ 28.77万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-15 至 2003-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2803525
• 关键词: bioimaging /biomedical imaging; biomedical equipment; biomedical equipment purchase; cardiovascular imaging /visualization; echocardiography

Genetic manipulation has become a powerful tool for elucidation of physiologic and pathophysiologic mechanisms in all of biology and is specifically gaining a major foothold in investigations of cardiovascular function and structure. The central strategy in such work is the generation of transgenic animals which is most commonly done in mice for which extensive mapping of the genome makes this work feasible. However, this addressed this limitation by adaptation of echocardiographic equipment and techniques which permits comprehensive assessment of cardiac function during light sedation in mice. Work to date has encompassed studies of cardiac function in transgenic mice with knock-outs of creatine kinases, angiotensin receptors, and (with a hypertrophic cardiomyopathy phenotype); and in rats with increased ventricular afterload induced by aortic banding. These studies have been done in the Pediatric Echocardiography and Hemodynamic Research Laboratory Facility of which the Director and Staff have been of enormous help in development of requisite procedures and conduct of the work. However, with the techniques now in hand, demand by investigators has risen steeply and access to equipment has become seriously limiting. Accordingly, this proposal seeks funding for a state-of-the-art echocardiographic unit uniquely suited for, and dedicated to, non- invasive in vivo acquisition and analysis of cardiac structure and function in small animals (mice and rats). The proposed equipment would serve a core group of seven investigators. The proposed equipment would sere a core group of seven investigators. The proposed unit would be housed in the Cardiovascular Pulmonary Research Laboratory and managed by dedicated staff and an advisory committee with ongoing advice and consultation with echocardiography personnel with whom the work to date has been conducted.

遗传操作已成为一个强大的工具，阐明生理和病理生理机制，在所有的生物学，特别是获得一个主要的立足点，在调查心血管功能和结构。这项工作的核心策略是产生转基因动物，这是最常见的在小鼠中进行的，对小鼠进行广泛的基因组作图使这项工作变得可行。然而，这解决了这一局限性的适应超声心动图设备和技术，允许在小鼠轻度镇静期间的心脏功能的全面评估。迄今为止的工作包括对肌酸激酶、血管紧张素受体和（肥厚型心肌病表型）基因敲除的转基因小鼠的心脏功能的研究;以及对主动脉结扎引起心室后负荷增加的大鼠的心脏功能的研究。这些研究是在儿科超声心动图和血液动力学研究实验室设施中完成的，其主任和工作人员在制定必要的程序和开展工作方面提供了巨大的帮助。然而，随着技术的掌握，调查人员的需求急剧增加，获得设备的机会受到严重限制。因此，该提案寻求为最先进的超声心动图装置提供资金，该装置特别适合并专用于小动物（小鼠和大鼠）心脏结构和功能的无创体内采集和分析。拟议的设备将为7名核心调查员提供服务。拟议的设备将用于一个由7名调查员组成的核心小组。拟议的单位将设在心血管肺研究实验室，由专职工作人员和咨询委员会管理，并与迄今为止开展工作的超声心动图工作人员不断提供建议和咨询。