MULTIUSER BIOLOGICAL TRANSMISSION ELECTRON MICROSCOPE

多用户生物透射电子显微镜

• 批准号: 2803453
• 负责人: Pietro De Camilli
• 金额: $ 36.29万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2803453
• 关键词: bioimaging /biomedical imaging; biomedical equipment; biomedical equipment purchase; digital imaging; transmission electron microscopy

Electron microscopy is a technique of fundamental importance in molecular and cell biology. Most of what we know about cell ultrastructure was learned from electron microscopy. Recently, several advancements have made possible he use of electron microscopy to obtain insight which go beyond descriptive information and the electron microscope has become a powerful tool to study molecular mechanisms. In large part, these developments reflect the advent of highly sensitive immunocythochemical techniques, of genetic methods to disrupt the function of individual proteins, and of cell free systems which make possible the analysis of isolated organelles or macromolecular complex. Furthermore, the advancements in cryoelectron microscopy are narrowing the gap between classical cell biology and structural biology. This application proposes the purchase of new transmission electron microscope (Philips CM120 BioTwin) and supporting equipment digital imaging. This instrumentation will be housed in the electron microscopy unit of the Center for Cell Imaging at the Yale School of Medicine. This is a shared facility, which is managed by the Department of Cell biology, but is subsidized by the School. It is used primarily by faculty from the Department of Cell Biology and other Departments whose research focuses on molecular and cell biology. The instrument will replace a Jeol 100cX which has been recently taken out of service The Yale Electron Microscopy Core Facility has traditionally been among the leaders in the application of electron microscopy to biology and medicine. While this continues to be the case, progress is now being hampered by microscope availability and by the outmoded instrumentation present in the facility. There have been major advances in electron microscope design and image digitalization and the facility is in urgent need of a state-of-the-art microscope. The productivity of Yale Cell Biology is crucially dependent on the availability of such an instrument.

电子显微镜是一种重要的技术， 分子和细胞生物学。 我们对细胞的大部分了解 电镜观察超微结构。 最近， 几项进步使电子显微镜的应用成为可能 获得超越描述性信息的洞察力， 电子显微镜已成为研究分子生物学的有力工具 机制等 在很大程度上，这些发展反映了 高度敏感的免疫细胞化学技术，遗传学方法 破坏单个蛋白质的功能， 能够分析分离的细胞器的系统，或 大分子复合物 此外， 冷冻电子显微镜正在缩小经典细胞之间的差距 生物学和结构生物学。 本申请建议购买新的透射电子 显微镜（Philips CM120 BioTwin）和配套设备 显像 这个仪器将被安置在电子 耶鲁大学医学院细胞成像中心的显微镜单元 药 这是一个共享设施，由 细胞生物学系，但由学校资助。 是 主要由细胞生物学系的教师使用， 其他研究重点在分子和细胞的部门 生物学该仪器将取代Jeol 100cX， 最近退出服务 耶鲁电子显微镜核心设施传统上一直是 在电子显微镜应用的领导者中， 生物学和医学。 虽然情况仍然如此，但进展 现在正受到显微镜可用性和过时的 设施内的仪器。 发生了重大 电子显微镜设计和图像数字化的进展， 该机构急需最先进的显微镜 的 耶鲁细胞生物学的生产力关键取决于 这样一个工具的可用性。