TOMOGRAPHY OF ENDOCYTIC INTERMEDIATES IN NERVE TERMINALS

神经末梢内吞中间体的断层扫描

• 批准号: 7955052
• 负责人: Pietro De Camilli
• 金额: $ 1.07万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955052
• 关键词: Cell membrane; Cellular Structures; Clathrin; Complex; Computer Retrieval of Information on Scientific Projects Database; Dynamin; Dynamin I; Endocytosis; Funding; Grant; Guanosine; Institution; Mus; Nerve; Neurons; Reaction; Research; Research Personnel; Resources; Source; Stimulus; Synapses; Synaptic Vesicles; Three-dimensional analysis; Tubular formation; United States National Institutes of Health; coated pit; electron tomography; mutant; postnatal; tomography

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dynamin 1 is a neuron-specific guanosine triphosphatase thought to be critically required for the fission reaction of synaptic vesicle endocytosis. Unexpectedly, mice lacking dynamin 1 were able to form functional synapses, even though their postnatal viability was limited. However, during spontaneous network activity, complex tubular vesicular invaginations were observed in synapses of dynamin 1knockout mice. A 3-D analysis using serial, electron tomography further revealed that branched, tubular plasma membrane invaginations accumulated in these mutant synapses, and these were capped by clathrin-coated pits. Synaptic vesicle endocytosis was severely impaired during strong exogenous stimulation but resumed efficiently when the stimulus was terminated. Thus, dynamin 1independent mechanisms can support limited synaptic vesicle endocytosis, but dynamin 1 is needed during high levels of neuronal activity.

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