IMAGE PLATE DETECTOR AND X RAY GENERATOR

图像板探测器和 X 射线发生器

• 批准号: 2766458
• 负责人: LEONARD J. BANASZAK
• 金额: $ 25.48万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-15 至 2000-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2766458
• 关键词: X ray; X ray crystallography; bioimaging /biomedical imaging; biomedical equipment purchase; cryoscience; radiation detector; synchrotrons

The Protein Crystallography Division of the Structural Biology Center at the University of Minnesota requests funds to purchase an x-ray generator and an image plate detector. The Structural Biology Center services investigators on two campuses from at least seven departments. Five faculty members in four departments use the resources and x-ray crystallography as their principal tool for structure:function studies. Currently 15 federally funded research programs are directly dependent on the x-ray diffraction equipment. The present instrumentation is nearly ten years old and must be replaced in the immediate future. In a number of studies, the existing equipment does not provide the resolution limits required by the project and more than half of the simultaneously appearing reflections are not recorded. The University of Minnesota established the Protein Crystallography Resource in 1989 with funds from the Minnesota Medical Foundation and from the University's operating funds. No NIH or other federal funds were used. During the last decade, monetary sources from the University of Minnesota have made possible the further development of the Center by providing a new suite of laboratories and high-powered computing resources for especially difficult crystallographic problems. In addition, most of the present graphics workstations used by the crystallographic staff have been purchased with University funds. The current resources have produced a number of important advances in areas such as lipid:protein interactions, metalloenzymes, iron storage proteins, citric acid cycle enzymes, microbial toxins and metabolic control of enzymes. At the present time and including post-doctoral fellows and graduate students, approximately twenty scientists use the x-ray resources as part of their ongoing research. In addition, a number of collaborative projects will need access to the x-ray diffraction instrumentation.

明尼苏达大学结构生物学中心的蛋白质晶体学部门要求资金购买X射线发生器和图像板探测器。 结构生物学中心为来自至少七个部门的两个校区的调查人员提供服务。四个系的五名教师使用这些资源和X射线晶体学作为他们结构的主要工具：功能研究。目前有15个联邦资助的研究项目直接依赖于X射线衍射设备。 目前的仪器已使用近十年，必须在不久的将来更换。 在一些研究中，现有设备不能提供项目所需的分辨率极限，同时出现的反射中有一半以上没有记录。明尼苏达大学于1989年建立了蛋白质晶体学资源，资金来自明尼苏达医学基金会和大学的运营基金。 没有使用NIH或其他联邦基金。 在过去的十年中，来自明尼苏达大学的资金来源使该中心的进一步发展成为可能，为特别困难的晶体学问题提供了一套新的实验室和高性能的计算资源。 此外，结晶学工作人员目前使用的大多数图形工作站都是用大学资金购买的。目前的资源已经在诸如脂质：蛋白质相互作用、金属酶、铁储存蛋白、柠檬酸循环酶、微生物毒素和酶的代谢控制等领域取得了一些重要进展。 目前，包括博士后研究员和研究生在内，大约有20名科学家使用X射线资源作为他们正在进行的研究的一部分。 此外，一些合作项目将需要获得X射线衍射仪器。