STRUCTURAL STUDIES OF LIPID/PROTEIN SYSTEMS

脂质/蛋白质系统的结构研究

• 批准号: 6594561
• 负责人: LEONARD J. BANASZAK
• 金额: $ 0.48万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6594561
• 关键词: X ray crystallography; acyl coA dehydrogenases; apolipoproteins; binding proteins; calorimetry; chemical kinetics; conformation; crystallization; fatty acid binding protein; fatty acid transport; intermolecular interaction; ionization constant; lipid transport; microsomes; nonblood lipoprotein; protein binding; protein disulfide isomerase; protein structure; retinoid binding proteins; sterol esterase; structural biology; thermodynamics; transport proteins; triglycerides; vitellin

DESCRIPTION (Adapted from abstract): The long-term objective is to provide data on the interaction of lipids with transport proteins or enzymes. To do this, the molecular structure of lipid-containing macromolecules will be used to describes lipid-protein interactions. A nearly complete study of lipovitellin has led to similar studies of microsomal triglyceride transfer protein (MTP), which is involved in assembly of the serum lipoproteins. A major aim is to compete the crystal structure of a complex of MTP with protein disulfide isomerase. For lipovitellin, new x-ray measurements have provided preliminary evidence about the conformation of 20 bound acyl chains. The new data will be refined and an effort will be made to reconstruct the entire domain of phospholipids present in lipovitellin. Attempts to crystallize apo A1, the principal protein component of HDL will be continued. In the beta-oxidation spiral, more than one enzyme appears to be associated with each reaction. For the beta-OH to beta-ketoacyl CoA reaction, humans have two different enzymes: one for short chain L-3-hydroxyacyl CoA substrates (SCHAD) and one for long chain metabolites (LCHAD). Preliminary x-ray data have identified the coenzyme site of SCHAD. The important goal now is to characterize the molecular structure that defines the origin of fatty acid chain specificity. This will be done by x-ray analyses of SCHAD associated with appropriate substrates. In a second stage, the LCHAD segment of a multifunctional protein will be expressed in E. coli, crystallized, and the 3D structure determined by diffraction methods. In a continuation of structural studies of intracellular proteins for shuttling fatty acids and retinoids, mutagenesis, titration calorimetry, and x-ray crystallography will be used to explore ligand specificity. One approach will combine experimental determination of proton binding with electrostatic calculations of characteristic pKs.

描述（改编自摘要）：长期目标是提供数据

项目成果

Crystallization of rat cellular retinol binding protein II. Preliminary X-ray data obtained from the apoprotein expressed in Escherichia coli.

大鼠细胞视黄醇结合蛋白 II 的结晶。

• 发表时间: 1987
• 期刊: The Journal of biological chemistry
• 作者: Sacchettini,JC;Stockhausen,D;Li,E;Banaszak,LJ;Gordon,JI
• 通讯作者: Gordon,JI

The adipocyte lipid-binding protein at 1.6-A resolution. Crystal structures of the apoprotein and with bound saturated and unsaturated fatty acids.

脂肪细胞脂质结合蛋白的分辨率为 1.6-A。

• 发表时间: 1993
• 期刊: The Journal of biological chemistry
• 作者: Xu,Z;Bernlohr,DA;Banaszak,LJ
• 通讯作者: Banaszak,LJ

Structure of the lamprey yolk lipid-protein complex lipovitellin-phosvitin at 2.8 A resolution.

七鳃鳗卵黄脂质-蛋白质复合物脂卵黄蛋白-卵黄高磷蛋白的结构，分辨率为 2.8 A。

• DOI: 10.1016/0022-2836(88)90542-6
• 发表时间: 1988
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Raag,R;Appelt,K;Xuong,NH;Banaszak,L
• 通讯作者: Banaszak,L

Lipid domains in the crystalline lipovitellin/phosvitin complex: a phosphorus-31 and deuterium nuclear magnetic resonance study.

结晶卵黄蛋白/卵黄高磷蛋白复合物中的脂质域：磷 31 和氘核磁共振研究。

• DOI: 10.1021/bi00539a024
• 发表时间: 1982
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Banaszak,LJ;Seelig,J
• 通讯作者: Seelig,J

Lipid environments in the yolk lipoprotein system. A spin-labeling study of the lipovitellin/phosvitin complex from Xenopus laevis.

卵黄脂蛋白系统中的脂质环境。

• DOI: 10.1021/bi00539a025
• 发表时间: 1982
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Birrell,GB;Anderson,PB;Jost,PC;Griffith,OH;Banaszak,LJ;Seelig,J
• 通讯作者: Seelig,J