QUANTITATIVE BIOCHEMICAL ANALYSIS OF MORPHOLOGICAL STRUCTURES IN HUMAN ARTERY

人体动脉形态结构的定量生化分析

• 批准号: 6121227
• 负责人: RIK BUSHMAN
• 金额: $ 2.04万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-01 至 2000-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121227
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; biomedical resource; cardiovascular system; human tissue; microscopy; spectrometry; technology /technique

The chemical and morphologocal composition of arterial wall change dramatically during plaque development. We have used 830-nm excited confocal Raman microscopy to collect in situ spectra of various chemical and morphological structures. So far, we have examined spectra of internal and external elastic lamina, collagen fibers, foam cells, necrotic core, adventitial lipid, cholesterol crystals, and calcium mineralization. These structures were studied in 35 different coronary artery samples representing different stages of atherosclerosis. Using these lineshapes, we are currently modeling bulk spectra to quantitatively extract the distribution of these structures. This in turn will be used to provide quantitative pathological information.

动脉壁的化学和形态组成改变 在牙菌斑形成过程中发生了显著变化。 我们使用830 nm激发 共焦拉曼显微镜，以收集各种 化学和形态结构。 到目前为止，我们已经检查了 内外弹性膜、胶原纤维、泡沫的光谱 细胞、坏死核心、外膜脂质、胆固醇晶体，以及 钙矿化 这些结构在35个不同的 代表不同阶段的冠状动脉样本 动脉粥样硬化 利用这些线条，我们目前正在模拟 批量光谱定量提取这些分布 结构. 这反过来将用于提供定量的 病理信息