QUANTITATIVE BIOCHEMICAL ANALYSIS OF MORPHOLOGICAL STRUCTURES IN HUMAN ARTERY

人体动脉形态结构的定量生化分析

• 批准号: 6281768
• 负责人: RIK BUSHMAN
• 金额: $ 1.89万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281768
• 关键词: biomedical equipment development; biomedical resource; cardiovascular system; human tissue; microscopy; spectrometry; technology /technique

A confocal Raman spectroscopy was developed with 830 nm laser excitation. High Raman spectra of various morphological structures in arterial wall such as elastic lamina, collagen fibers, adventitial fat, cholesterol crystals, foam cells and calcium mineralization. The objective the present estimate quantitative biochemical composition of each of the morphological structures present in tissues. We used a least square minimization routine to model spectra of morphological structure in terms of nine biochemical such as collagen, elastin, triolein, cholesterol, cholesterol linoleate, phosphatidyl choline, b-carotene, calcium hydroxy apatite, and calcium carbonate. Near perfect fit were obtained for each morphological structure studied and chemical composition was determined. Comparison of these results provided detailed information about intra and inter structural chemical variations at different stages of atherosclerosis.

研制了830 nm激光共聚焦拉曼光谱。 激动人心。不同形态结构的高拉曼光谱 动脉壁如弹力板、胶原纤维、外膜 脂肪、胆固醇晶体、泡沫细胞和钙矿化。这个 目的目前估计的定量生化成分。 每一种形态结构都存在于组织中。我们使用了一个 形态光谱建模的最小二乘算法 结构在九种生化方面，如胶原，弹性蛋白， 三酸甘油酯，胆固醇，胆固醇亚油酸盐，磷脂酰胆碱， B-胡萝卜素、羟基磷灰石和碳酸钙。附近 对所研究的每个形态结构都获得了完美的匹配，并 测定了其化学成分。这些结果的比较 提供了有关内部和内部结构的详细信息 动脉粥样硬化不同阶段的化学变化。