QUANTITATIVE BIOCHEMICAL ANALYSIS OF MORPHOLOGICAL STRUCTURES IN HUMAN ARTERY

人体动脉形态结构的定量生化分析

• 批准号: 6314199
• 负责人: RIK BUSHMAN
• 金额: $ 1.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314199
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; biomedical resource; cardiovascular system; human tissue; microscopy; spectrometry; technology /technique

A confocal Raman spectroscopy was developed with 830 nm laser excitation. High Raman spectra of various morphological structures in arterial wall such as elastic lamina, collagen fibers, adventitial fat, cholesterol crystals, foam cells and calcium mineralization. The objective the present estimate quantitative biochemical composition of each of the morphological structures present in tissues. We used a least square minimization routine to model spectra of morphological structure in terms of nine biochemical such as collagen, elastin, triolein, cholesterol, cholesterol linoleate, phosphatidyl choline, b-carotene, calcium hydroxy apatite, and calcium carbonate. Near perfect fit were obtained for each morphological structure studied and chemical composition was determined. Comparison of these results provided detailed information about intra and inter structural chemical variations at different stages of atherosclerosis.

采用830 nm激光共焦拉曼光谱技术 激发 高拉曼光谱的各种形态结构， 动脉壁如弹性膜、胶原纤维、外膜 脂肪、胆固醇结晶、泡沫细胞和钙矿化。 的 目的定量测定 存在于组织中的每种形态结构。 我们使用了 最小二乘最小化程序来模拟形态学谱 胶原蛋白，弹性蛋白， 三油酸甘油酯，胆固醇，胆固醇亚油酸酯，磷脂酰胆碱， β-胡萝卜素、羟基磷灰石钙和碳酸钙。 附近 对于所研究的每种形态学结构获得了完美的拟合， 确定了化学成分。 这些结果的比较 提供了有关内部和内部结构的详细信息， 动脉粥样硬化不同阶段的化学变化。