LOCALIZATION OF ANTIBODIES TO OXIDIZED LDL IN NORMAL & APOPTOTIC CELLS

正常情况下氧化 LDL 抗体的定位

• 批准号: 6220682
• 负责人: Joseph L. Witztum
• 金额: $ 3.85万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-15 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6220682
• 关键词: biomedical resource; clinical research; female; human subject; microscopy; neoplasm /cancer; reproductive system; women's health

We have shown through immunofluorescence and confocal microscopy that centrosomes of high grade breast tumors are larger and more numerous than centrosomes in normal breast tissue. Additionally, the centrosomes in breast tumor cells are hyperphosphorylated and are capable of nucleating a larger number of microtubules in vitro as compared to centrosomes from normal breast cells. We describe these centrosomes as "hypertrophic". Attempts to characterize hypertrophic centrosomes at an ultrastructural level using CTEM have been difficult. This is due, at least in part, to the small volume of the centrosomes relative to the total volume of the cells; the centrosomes are few and far between in 90 nm thick sections through cells that are 6-10 um thick. In addition, the hypertrophic centrosomes we have observed with CTEM have been especially electron opaque, making it difficult to resolve microtubules and other structural components of the centrosomes. Initial results using 0.5 and 1 micron thick sections have proven not only to greatly facilitate the locating of hypertropic centrosomes, but have also produced 3-dimensional images with heretofore unseen structural detail. Several tomographic reconstructions of centrosomes from cancerous cells were performed when Dr. Lingle visited the laboratory last summer. Additional reconstructions are planned in the near future.

我们通过免疫荧光和共聚焦显微镜 高级别乳腺肿瘤的中心体更大， 比正常乳腺组织中的中心体多。 另夕h 乳腺肿瘤细胞中的中心体被过度磷酸化， 能够在体外使大量微管成核， 与正常乳腺细胞的中心体相比。 描述此等 中心体“肥大”。 试图描述肥大性 使用CTEM在超微结构水平上观察中心体， 难 这是由于，至少部分是由于， 相对于细胞总体积的中心体;中心体 在90 nm厚的切片中， 6-10嗯厚的。 另外，我们发现的肥大中心体 用CTEM观察到的是电子不透明的， 很难分辨微管和其他结构成分 中心体 使用0.5和1微米厚的初始结果 事实证明，不仅大大方便了 肥大的中心体，但也产生了三维图像 有着前所未有的结构细节 几个断层扫描 进行癌细胞中心体的重建 林格尔博士去年夏天访问实验室时 额外 计划在不久的将来进行重建。