PPG Phenotyping

项目摘要 核心A。表型 表型核心A的使命是提供标准化的分析方法和服务， 协助项目研究人员有效地实现各自项目的科学目标。核心 将提供小鼠模型和人类受试者的靶向表型分析，以及提供选择的 项目使用的试剂。核心分为5个子核心，将提供以下服务： 1)一个动脉粥样硬化形态子核心，提供小鼠的定性和定量分析 动脉粥样硬化2)肝脏形态子核心，提供小鼠肝脏的定性和定量分析 在NASH和纤维化的背景下的组织学。3)靶向脂质组学分析子核心， UCSD LIPID MAPS Lipidomic Core中的血浆和组织。4)有针对性的蛋白质组亚核心， 使用Luminex生物分析仪，可对细胞因子、趋化因子和其他分析物进行高通量、高灵敏度分析， 5）。脂蛋白亚核心，将测量小鼠血浆中的脂质和脂蛋白水平 还提供LDL和修饰的LDL（和其他所需的脂蛋白）的标准化制剂， 调查人员的使用。 核心将集中在加州大学圣地亚哥分校的Witztum博士的实验室，他将是整体 负责人和负责人，以确保所有的子核心完成他们的使命。动脉粥样硬化形态学 和肝脏形态子核心将在Witztum博士的指导下在以下人员的协助下存放在UCSD 加州大学圣地亚哥分校肝脏病理学专家（Tatiana Kisseleva博士），以及脂蛋白核心的服务。高- 细胞因子、趋化因子和相关蛋白的通量选择蛋白质组类型分析将在 索尔克研究所在罗恩埃文斯和迈克尔唐斯博士的指导下。最后，Lipid Maps Lipidomic 核心，在博士的指导下。Oswald Quehenberger和艾德丹尼斯也位于加州大学圣地亚哥分校， 是维茨顿博士的实验室因此，所有样本采集将集中在中心实验室， UCSD，在那里他们将被记录到Excel电子表格中，为以下活动提供中央组织途径： 将样本分配到适当的子核心，并反过来，用于结果数据收集的中心站点。

PROJECT SUMMARY Core A. Phenotyping The mission of the Phenotyping Core A is to provide standardized analytical methodologies and services to assist the Project Investigators in efficiently achieving the Scientific Aims of their respective projects. The Core will provide targeted phenotyping of both mouse models and human subjects, as well as providing selected reagents used by the Projects. The Core is organized into 5 Sub-Cores, which will provide the following services: 1) An Atherosclerosis Morphology Sub-Core to provide qualitative and quantitative analyses of mouse atherosclerosis. 2) Liver Morphology Sub-Core to provide qualitative and quantitative analysis of mouse liver histology in the context of NASH and fibrosis. 3) Targeted Lipidomic Analysis Sub-Core to provide analyses of plasma and tissues in the UCSD LIPID MAPS Lipidomic Core. 4) Targeted Proteomic Sub-Core to provide high- throughput, high- sensitivity analysis of cytokines, chemokines and other analytes with the use of Luminex Bio- Plex technology and 5). Lipoprotein Sub-Core, which will measure lipid and lipoprotein levels in mouse plasma and also provide standardized preparations of LDL and modified LDLs (and other needed lipoproteins) for investigators’ use. The Core will be centered and organized in Dr. Witztum's laboratory at UCSD, who will be the overall director and responsible to see that all of the Sub-Cores fulfill their missions. The Atherosclerosis Morphology and Liver Morphology Sub-Cores will be housed at UCSD under the Direction of Dr. Witztum with assistance of a UCSD expert in liver pathology (Dr. Tatiana Kisseleva), as will the services of the Lipoprotein Core. High- throughput selected proteomic type analyses of cytokines, chemokines and related proteins will be performed at the Salk Institute under the direction of Drs. Ron Evans and Michael Downes. Finally, the Lipid Maps Lipidomic Core, under the direction of Drs. Oswald Quehenberger and Ed Dennis is also located at UCSD in the same building as Dr. Witztum’s laboratories. Thus, all sample collection will be centralized in a central laboratory at UCSD, where they will be logged into an Excel spreadsheet to provide a central organizational pathway for distribution of samples to proper Sub-Cores and in turn, a central site for data collection of results.