PPG Phenotyping

项目总结 核心A.表型 表型分析核心A的任务是提供标准化的分析方法和服务 协助项目调查人员有效地实现各自项目的科学目标。《核心》 将提供对小鼠模型和人类受试者的有针对性的表型分析，以及提供选定的 项目使用的试剂。核心由5个子核组成，将提供以下服务： 1)提供对小鼠的定性和定量分析的动脉粥样硬化形态亚核 动脉硬化。2)肝脏形态子核，提供对小鼠肝脏的定性和定量分析 NASH和纤维化背景下的组织学。3)有针对性的脂组分析分核心，以提供以下分析 UCSD中的血浆和组织的脂类核心图。4)有针对性的蛋白质亚核，以提供高- 使用Luminex Bio-2进行细胞因子、趋化因子和其他分析物的高灵敏度分析。 Plex技术和5)。脂蛋白亚核，它将测量小鼠血浆中的脂质和脂蛋白水平 还提供标准化的低密度脂蛋白和修饰的低密度脂蛋白(以及其他所需的脂蛋白) 调查人员的使用。 核心中心将在加州大学圣迭戈分校的维茨塔姆博士的实验室中进行集中和组织，他将是整个 主管并负责确保所有分核完成各自的任务。动脉粥样硬化的形态学研究 和肝脏形态分中心将在威茨图姆博士的指导下在加州大学圣迭戈分校 加州大学圣迭戈分校肝脏病理学专家(Tatiana Kisseleva博士)，以及脂蛋白核心的服务。高- 通过选定的细胞因子、趋化因子和相关蛋白质的蛋白质组类型分析将在 罗恩·埃文斯博士和迈克尔·唐斯博士领导下的索尔克研究所。最后，类脂映射类脂组学 Core，在Oswald Quehenberger博士和Ed Dennis的指导下，也位于加州大学圣地亚哥分校的同一 作为维茨塔姆博士的实验室。因此，所有样本收集将集中在一个中心实验室。 UCSD，他们将在那里登录到Excel电子表格，以提供中央组织路径 将样品分发到适当的分岩心，进而成为收集结果数据的中央站点。

PROJECT SUMMARY Core A. Phenotyping The mission of the Phenotyping Core A is to provide standardized analytical methodologies and services to assist the Project Investigators in efficiently achieving the Scientific Aims of their respective projects. The Core will provide targeted phenotyping of both mouse models and human subjects, as well as providing selected reagents used by the Projects. The Core is organized into 5 Sub-Cores, which will provide the following services: 1) An Atherosclerosis Morphology Sub-Core to provide qualitative and quantitative analyses of mouse atherosclerosis. 2) Liver Morphology Sub-Core to provide qualitative and quantitative analysis of mouse liver histology in the context of NASH and fibrosis. 3) Targeted Lipidomic Analysis Sub-Core to provide analyses of plasma and tissues in the UCSD LIPID MAPS Lipidomic Core. 4) Targeted Proteomic Sub-Core to provide high- throughput, high- sensitivity analysis of cytokines, chemokines and other analytes with the use of Luminex Bio- Plex technology and 5). Lipoprotein Sub-Core, which will measure lipid and lipoprotein levels in mouse plasma and also provide standardized preparations of LDL and modified LDLs (and other needed lipoproteins) for investigators’ use. The Core will be centered and organized in Dr. Witztum's laboratory at UCSD, who will be the overall director and responsible to see that all of the Sub-Cores fulfill their missions. The Atherosclerosis Morphology and Liver Morphology Sub-Cores will be housed at UCSD under the Direction of Dr. Witztum with assistance of a UCSD expert in liver pathology (Dr. Tatiana Kisseleva), as will the services of the Lipoprotein Core. High- throughput selected proteomic type analyses of cytokines, chemokines and related proteins will be performed at the Salk Institute under the direction of Drs. Ron Evans and Michael Downes. Finally, the Lipid Maps Lipidomic Core, under the direction of Drs. Oswald Quehenberger and Ed Dennis is also located at UCSD in the same building as Dr. Witztum’s laboratories. Thus, all sample collection will be centralized in a central laboratory at UCSD, where they will be logged into an Excel spreadsheet to provide a central organizational pathway for distribution of samples to proper Sub-Cores and in turn, a central site for data collection of results.