MULTIDIMENSIONAL LC & TANDEM MASS SPECTROMETRY ANALYSIS OF TRANSLATION COMPLEX

多维液相色谱

• 批准号: 6206577
• 负责人: JOHN R YATES
• 金额: $ 0.43万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6206577
• 关键词: bioengineering /biomedical engineering; biomedical resource; genome; microorganism; plants; proteins; technology /technique

Screens of the yeast activation domain array have been proposed for a number of Gal4 DNA-binding domain fusions constructed from products of genes involved in the DNA damage checkpoint. Screens of Rad9, Rad17, Rad53, and Mec3 are now under way. We have also constructed Gal4-DNA binding domain hybrids for several other checkpoint gene products including Pds1, Mec1, Dun1, Pol30, and Rad24 which we plan to use in array screens.

酵母激活结构域阵列的筛选已被提出 对于许多Gal 4 DNA结合结构域融合体， 与DNA损伤检查点有关的基因产物。 屏幕 Rad 9，Rad 17，Rad 53和Mec 3正在进行中。 我们还 构建了Gal 4-DNA结合结构域杂交体， 检查点基因产物包括Pds 1、Mec 1、Dun 1、Pol 30和Rad 24 我们计划在阵列屏幕中使用它。