REGULATION OF ARP2/3 COMPLEX ACTION IN CELL MOTILITY

细胞运动中 ARP2/3 复杂作用的调节

• 批准号: 6178852
• 负责人: HENRY N HIGGS
• 金额: $ 4.09万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6178852
• 关键词: cell motility; cytoskeletal proteins; phosphorylation; protein sequence; protein structure function; site directed mutagenesis

The long-term goal of the proposed research is to understand the mechanisms involved in cellular motility. The ability of cells to move is central to a myriad of physiological processes including embryonic development, neural network formation, immune function, and metastisis. The project focuses on a multi-protein complex, the Arp2/3 complex, that appears to play a central role in lamellipodial cell motility. Initial studies will address the mechanisms by which Arp2/3 function is regulated, with particular attention to phosphorylation. These studies will test the effects of phosphorylation both in mammalian cells and in cell-free assays. Subsequent studies will focus on the pathways by which this regulation occurs, with focus on Rho family GTPases. In addition, Arp2/3 complex interacting factors will be identified. Finally, the reasons for the existence of isoforms for one of the Arp2/3 complex subunits will be addressed.

拟议研究的长期目标是了解 参与细胞运动的机制。 细胞移动的能力 是包括胚胎在内的无数生理过程的核心 发育、神经网络形成、免疫功能和转移。 该项目重点关注多蛋白复合物，Arp2/3 复合物， 似乎在板状足细胞运动中发挥核心作用。 最初的 研究将探讨 Arp2/3 功能的机制 调节，特别注意磷酸化。 这些研究 将测试磷酸化在哺乳动物细胞和 无细胞测定。 后续研究将重点关注以下途径 这种调节的发生主要集中在 Rho 家族 GTPases 上。在 此外，Arp2/3 复杂的相互作用因素也将被确定。 最后，Arp2/3之一存在异构体的原因 将解决复杂的子单元。