Regulatory mechanisms of cytoskeletal proteins by phosphorylation and their cellular functions

细胞骨架蛋白磷酸化的调控机制及其细胞功能

• 批准号: 16370092
• 负责人: INAGAKI Masaki
• 金额: $ 10.11万
• 依托单位: Aichi Cancer Center (Research Institute)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16370092/
• 关键词: phosphorylation; intermediate filament; cyclin-dependent kinase; Plk1; Aurora-B; INCENP; Trichoplein; Fbf-1; リン酸化反応; 細胞骨格; 細胞周期; 細胞極性; トリコプレイン; Fbf1; ケラチン

We found that Cdk1 phosphorylates vimentin-Ser55 from prometaphase to metaphase, leading to the recruitment of Plk-1 to vimentin. Upon binding to Phospho-Ser55 of vimentin, Plk1 is activated, and then phosphorylates vimentin-Ser82. During cytokinesis, Rho-kinase and Aurora-B specifically phosphorylate IFs at the cleavage furrow. The IF phosphorylation by Cdk1, Plk1, Rho-kinase, and Aurora-B plays an important role in the local IF breakdown, and is essential for the efficient segregation of IF networks into daughter cells. We also. found that Cdk1 phosphorylates Thr59 and Thr388 on inner centromere protein (lNCENP). INCENP phosphorylation by Cdk1 is necessary for the recruitment of Plk1 to the kinetochore, and the complex formation of Plk1 and Aurora-B on INCENP may play crucial roles in the regulation of chromosome dynamics. We identified Trichoplein and Fas binding factor-1 (Fbf-I) as novel keratin-binding proteins. Trichoplein and Fbf-1 have a domain that shows a low degree of sequence similarity between trichohyalin and plectin, designated, as trichohyalin/plectin homology domain (TPHD). These proteins co-localized with keratin, and also localized at cell-cell adhesion.

我们发现Cdk 1磷酸化波形蛋白-Ser 55从前中期到中期，导致招聘的Plk-1波形蛋白。当与波形蛋白的磷酸化丝氨酸55结合时，Plk 1被激活，然后磷酸化波形蛋白-丝氨酸82。在胞质分裂过程中，Rho激酶和Aurora-B特异性磷酸化裂解沟处的IFs。由Cdk 1、Plk 1、Rho激酶和Aurora-B引起的IF磷酸化在局部IF分解中起重要作用，并且对于IF网络有效分离成子细胞至关重要。我们也是。发现Cdk 1磷酸化内着丝粒蛋白（INCENP）上的Thr 59和Thr 388。INCENP被Cdk 1磷酸化是Plk 1向动粒募集所必需的，并且Plk 1和Aurora-B在INCENP上的复合物形成可能在染色体动力学的调节中起关键作用。我们确定Trichoplein和Fas结合因子-1（Fbf-I）为新的角蛋白结合蛋白。Trichoplein和Fbf-1具有一个结构域，该结构域在透明质蛋白和纤维素之间显示出低程度的序列相似性，称为透明质蛋白/纤维素同源结构域（TPHD）。这些蛋白质与角蛋白共定位，并且也定位于细胞-细胞粘附。

项目成果

Vimentin‐Ser82 as a memory phosphorylation site in astrocytes

• DOI: 10.1111/j.1365-2443.2006.00961.x
• 发表时间: 2006-05
• 期刊: Genes to Cells
• 影响因子: 2.1
• 作者: Takashi Oguri;Akihito Inoko;H. Shima;I. Izawa;N. Arimura;Tomoya Yamaguchi;N. Inagaki;K. Kaibuchi;K. Kikuchi;M. Inagaki

Vimentin rearrangement during African swine fever virus infection involves retrograde transport along microtubules and phosphorylation of vimentin by calcium calmodulin kinase II

• DOI: 10.1128/jvi.79.18.11766-11775.2005
• 发表时间: 2005-09-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Stefanovic, S;Windsor, M;Wileman, T
• 通讯作者: Wileman, T

Aurora-B and Rho-kinase/ROCK, the two cleavage furrow kinases, independently regulate the progression of cytokinesis: possible existence of a novel cleavage furrow kinase phosphorylates ezrin/radixin/moesin (ERM)

• DOI: 10.1111/j.1365-2443.2005.00824.x
• 发表时间: 2005-02-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Yokoyama, T;Goto, H;Inagaki, M
• 通讯作者: Inagaki, M

Aurae-B and Rho-kinase/ROCK. the two cleavage furrow Kinnocks, independently the progression of pylokinooisl geseibte exleitnca of a novel claevboe furrow kinases phamphorylstan ezrinisodlkin/moemo (ERM).

Aurae-B 和 Rho 激酶/ROCK。

• 发表时间: 2005
• 期刊: Genes Cells 10
• 作者: Yokoyama;T. et al.
• 通讯作者: T. et al.

Cytoskeletal modification of Rho guanine nucleotide exchange factor acivity : identification of a Rho guanine nucleotide exchange factor as a binding partner for Sept9b, a mammalian septin.

Rho 鸟嘌呤核苷酸交换因子活性的细胞骨架修饰：鉴定 Rho 鸟嘌呤核苷酸交换因子作为 Sept9b（一种哺乳动物脓毒蛋白）的结合伴侣。

• 发表时间: 2005
• 期刊: Oncogene 24
• 作者: Nagata;K.;Inagaki;M.
• 通讯作者: M.