NONINVASIVE HIGH ENERGY PHOSPHATE IMAGING

无创高能磷酸盐成像

• 批准号: 6056609
• 负责人: PAUL A BOTTOMLEY
• 金额: $ 12.13万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-21 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6056609
• 关键词: adenosine triphosphate; bioimaging /biomedical imaging; creatine phosphate; high energy compound; human subject; imaging /visualization /scanning; magnetic resonance imaging; method development; nuclear magnetic resonance spectroscopy; phosphates

DESCRIPTION (Adapted from the applicant's abstract): Oxidative energy metabolism is essential for most cellular function including muscular contraction and ion transport. Phosphorus (31P) magnetic resonance spectroscopy (MRS) is unique in its ability to provide non-invasive access to key HEP metabolites such as adenosine triphosphate (ATP), the body's essential energy currency, and phosphocreatine (PCr), a key energy reserve. In the heart, clinical MRS shows reduced levels of PCr and ATP in infarction, and PCr/ATP ratios during ischemia. These and other MRS abnormalities seen in muscle disease, cancer, and the brain (e.g., stroke, some dementia), highlight the role of altered energy metabolism in common disease states, and its potential for evaluating therapeutic response. However, studies of HEPs access to deep-lying tissue, and the complexity of spectroscopic analysis. To address these critical problems, the investigators first demonstrated that the sensitivity of human HEP in the torso can be significantly increased by nuclear Overhauser enhancement (nOe) technique. Second, they separately showed that the use of (31)P phased-array coils can greatly increase sensitivity and access to PCr and ATP levels in the body. Third, to simplify MRS analysis, it has been demonstrated that HEP (PCr or PC and ATP) can be directly imaged in the body in the normoxic and ischemic conditions with chemical-selective (31)P MRI. The applicants now propose to combined phased-array with nOe technologies to provide critically important sensitivity gains of 3-fold and higher for (31)P studies of HEP in the body. They further propose that the combination of direct chemical-selective MRI of HEP will largely eliminate the analysis burden for MRS with phased-arrays. In addition, it is proposed that HEP imaging will further benefit from a priori spatial information acquired by routine proton MRI. The integration of these diverse new technologies presents new challenges requiring innovate solutions. Success in their integration will create a new biomedical research tool to non-invasively probe energy metabolism in human disease. Thus, the applicants propose to combine, for the first time, the sensitivity advantages provided by the physics technique of nOe with the engineering technique of phased arrays, chemical-selective MRI of medical imaging and new science analysis methods to create HEP imaging.

描述(改编自申请人的摘要)：氧化能 新陈代谢对包括肌肉在内的大多数细胞功能都是必不可少的。 收缩和离子运输。磷(31P)磁共振 光谱学(MRS)的独特之处在于它能够提供非侵入性的访问 对关键的HEP代谢物，如三磷酸腺苷(ATP)，身体的 基本能源货币和关键能源--磷酸肌酸 保留。在心脏，临床MRS显示 心肌梗死和缺血时的聚合酶链式反应/ATP比值。这些和其他的MRS 肌肉疾病、癌症和大脑的异常(例如，中风， 一些痴呆症)，突出了能量代谢改变的共同作用 疾病状态及其评估治疗反应的潜力。 然而，关于HEPS接触深层组织的研究，以及其复杂性 光谱分析。为了解决这些关键问题， 研究人员首先证明了人类HEP在体内的敏感性 躯干可以通过核Overhauser增强显著增加 (NOE)技术。其次，他们分别证明了(31)P的使用 相控阵线圈可以极大地提高灵敏度和获得PCR和 体内的三磷酸腺苷水平。第三，为了简化MRS分析，它被 证明了HEP(聚合酶链式反应或PC和ATP)可以直接在 具有化学选择性(31)P的常氧和缺血条件下的身体 核磁共振检查。申请者现在提议将相控阵与NOE相结合 技术可将极其重要的灵敏度提高3倍 对HEP在体内的(31)P研究较高。他们进一步提出， HEP的直接化学选择性磁共振结合将在很大程度上 利用相控阵消除了MRS的分析负担。此外，它还 提出HEP成像将进一步受益于先验空间 常规质子磁共振成像获得的信息。这些功能的整合 多样化的新技术带来了新的挑战，需要创新 解决办法。它们的成功整合将创造一种新的生物医学 非侵入性探测人类疾病能量代谢的研究工具。 因此，申请者首次提议将 NOE物理技术提供的灵敏度优势 医用相控阵、化学选择性磁共振成像工程技术 成像和新的科学分析方法，以创建HEP成像。