DERIVING NEW ORGANISMS BY CONSTRUCTING HYBRID GENOMES

描述（改编自研究者摘要）：拟 通过组合微生物的片段构建一系列杂交微生物， 将两个或多个生物体的基因组整合到一个微生物染色体中。 以前在这一领域的工作涉及将一个或几个基因引入到 高等细胞，例如构建转基因小鼠，或者 通过质粒载体将一个或几个基因引入微生物，所述质粒载体 留在细胞质中。 然而，这里建议创建新的 微生物通过引入大的（大于10个基因，以及多达 100到1，000个基因）的基因片段进入细胞， 通过基因工程直接植入染色体。 在许多情况下 构建的杂交体将具有一系列所需的性质， 不同的微生物，如固氮途径，浮油 降解途径和抗生素合成途径。 这些新物种 可以以有计划的，有指导的方式， 或者通过不同基因组片段的随机组合来开启一个时代 “组合基因组。"

DESCRIPTION (adapted from investigator's abstract): It is proposed to construct a series of hybrid microorganisms by combining segments of the genomes of two or more organisms into one single microorganism chromosome. Previous work in this area has involved bringing one or several genes into higher cells, such as the construction of a transgenic mouse, or else bringing one or several genes into a microorganism via a plasmid vector that remains in the cytoplasm. It is proposed here, however, to create new microorganisms by introducing large (greater than 10 genes, and as many as 100 to 1,000 genes) genetic segments into the cell and incorporating them directly into the chromosome by genetic engineering. In many cases the constructed hybrids will have a series of desired properties derived from different microorganisms, such as nitrogen fixation pathways, oil slick degradation pathways, and antibiotic synthesis pathways. These new species of microorganisms can be constructed in either a planned, directed manner, or by random combination of segments of different genomes to open up an era of "combinatorial genomes."