DERIVING NEW ORGANISMS BY CONSTRUCTING HYBRID GENOMES

通过构建混合基因组衍生新生物

• 批准号: 6019453
• 负责人: JEFFREY H MILLER
• 金额: $ 10.71万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6019453
• 关键词: Escherichia coli; aerobiosis; aminoacid biosynthesis; anaerobiosis; bacterial genetics; biotechnology; genetic manipulation; microorganism metabolism; plasmids; recombinant DNA; transfection /expression vector

DESCRIPTION (adapted from investigator's abstract): It is proposed to construct a series of hybrid microorganisms by combining segments of the genomes of two or more organisms into one single microorganism chromosome. Previous work in this area has involved bringing one or several genes into higher cells, such as the construction of a transgenic mouse, or else bringing one or several genes into a microorganism via a plasmid vector that remains in the cytoplasm. It is proposed here, however, to create new microorganisms by introducing large (greater than 10 genes, and as many as 100 to 1,000 genes) genetic segments into the cell and incorporating them directly into the chromosome by genetic engineering. In many cases the constructed hybrids will have a series of desired properties derived from different microorganisms, such as nitrogen fixation pathways, oil slick degradation pathways, and antibiotic synthesis pathways. These new species of microorganisms can be constructed in either a planned, directed manner, or by random combination of segments of different genomes to open up an era of "combinatorial genomes."

描述（改编自研究者的摘要）：建议 通过组合片段构建一系列杂交微生物 将两种或多种生物体的基因组整合到一条微生物染色体中。 该领域之前的工作涉及将一个或多个基因引入 高等细胞，例如转基因小鼠的构建，或者 通过质粒载体将一个或多个基因带入微生物， 保留在细胞质中。 不过，这里建议创建新的 通过引入大型（超过 10 个基因，并且多达 100 到 1,000 个基因）遗传片段进入细胞并将其整合 通过基因工程直接进入染色体。 在许多情况下 构建的混合体将具有一系列所需的特性，这些特性源自 不同的微生物，例如固氮途径、浮油 降解途径和抗生素合成途径。 这些新物种 微生物可以以有计划的、有指导的方式构建， 或者通过不同基因组片段的随机组合来开辟一个时代 “组合基因组”。