REPRODUCTIVE PARAMETERS OF CHIMPANZEE MODEL

最近的数据表明，EP1是一种胆固醇交换蛋白 它是由所有的上皮细胞合成和分泌的。因此， 我们集中精力确定EP2在人类免疫系统中的作用 受精过程。我们已经证明了EP2是一种 雄激素依赖的附睾特异性分泌蛋白，即 合成和分泌的特异地在头区域 黑猩猩的附睾体。我们已经确定了其他几种选择 EP2消息，我们还没有对其进行表征。此外， 黑猩猩的选择性剪接模式不同于 恒河猴。恒河猴使用几个额外的外显子来 抄写三个开放阅读框。为了更好地理解 拼接变体和它们的两个启动子，我们开始了基因组的工作 EP2克隆(PAK)。Pak(P1人工染色体)，具有插入大小 超过100kb，似乎包含完整的EP2基因。 初步分析，用聚合酶链式反应来桥接基因、指标 EP2基因至少包含10个外显子，其中5个外显子 在EP2A变种中，后3个在EP2B中使用 变种。我们已经证明EP1不与精子表面结合。 从射出的黑猩猩精子中提取的蛋白质蛋白质印迹显示 只有在低盐萃取物中才有染色强烈的成分。vbl.使用 免疫组织化学染色，我们显示EP1似乎是 合成和分泌主要由猪上皮细胞VI-VIII分泌 头中经远端头端-近端体部的第IX层上皮 黑猩猩附睾管的移行。资助美国国立卫生研究院 /RR0599407$165,627 4/15/97-8/14/01出版物*Smithwick，例如， 古尔德，K.G.和杨，L.G.免疫组织化学定位 成年雄性黑猩猩附睾分泌蛋白EP1的研究 狼人)。《组织与细胞》(印刷中)。杨氏，L.G.，弗罗里奇，O. 和古尔德，K.G.HE2/EP2，一种雄激素依赖的蛋白质 黑猩猩(黑猩猩)附精。J.Reprod.费特。(在 按下)。P51RR00165-38 1998年1月1日-1998年12月31日耶尔克斯地区灵长类 研究中心

Recent data indicates that EP1 is a cholesterol exchange protein and that it is synthesized and secreted by all epithelia. Therefore, we have concentrated our efforts on determining the role of EP2 in the fertilization process. We have shown that EP2 is an androgen-dependent, epididymis-specific secretory protein that is synthesized and secreted specifically in the caput region of the chimpanzee epididymis. We have identified several other alternative EP2 messages, which we have not yet characterized. Further, the alternative splicing pattern for chimpanzee is different from that of the rhesus monkey. The rhesus monkey uses several additional exons to transcribe the three open reading frames. To better understand the splicing variants and their two promoters, we began work on a genomic EP2 clone (PAK). PAK (P1 artificial chromosome), has an insert size of over 100 kb that appears to contain the entire EP2 gene. Preliminary analyses, using PCR to bridge the intr ons, indi cates that the EP2 gene contains at least 10 exons, five of which are used in the EP2A variant and the last 3 of which are used in the EP2B variant. We have shown that EP1 does not bind to the sperm surface. Western blots of proteins from ejaculated chimpanzee sperm showed intensely stained components only in low salt extract. Using immunohistochemical staining, we showed that EP1 appears to be synthesized and secreted primarily by epithelia VI-VIII of the mid-caput through epithelium IX of the distal caput-proximal corpus transition of the ductus epididymides of the chimpanzee. FUNDING NIH / RR0599407 $165,627 4/15/97 - 8/14/01 PUBLICATIONS *Smithwick, E.G., Gould, K.G. and Young, L.G. Immunohistochemical localization of epididymal secretory protein EP1 in the adult male chimpanzee (Pan troglodytes). Tissue and Cell (In press). *Young, L.G., Frolich, O. and Gould, K.G. HE2/EP2, an androgen dependent protein of the chimpanzee (Pan troglodytes) epididymis. J. Reprod. Fert. (In press). P51RR00165-38 1/1/98 - 12/31/98 Yerkes Regional Primate Research Center