DEVELOPMENT OF DNA VACCINES FOR CONTRACEPTION

这是一项尝试免疫男性和女性的先导性研究。 猕猴对雄激素依赖的附睾特异性蛋白的作用 与精子功能有关。其基本原理是免疫接种 雌性，随着抗体的分泌进入雌性生殖器 肠道，会干扰受精。与之相关的DNA 以EP2的表达为抗原，皮内给药 使用基因枪。在注射的八只雌性中，有两只表现出 抗体形成的证据。虽然有可能推迟怀孕，但 没有被抑制。未来的工作将针对优化 疫苗接种程序与DNA途径的改变 为了最大限度地提高抗体产量，在 生殖挑战。

This is a pilot study to attempt to immunize male and female macaques to an androgen dependent, epididymis specific protein associated with sperm function. The rationale is that immunization of the female, with secretion of antibody into the female reproductive tract, will interfere with fertilization. The DNA associated with expression of EP2 was used as antigen, administered intradermally using a gene gun. Of eight females injected, two demonstrated evidence of antibody formation. Although possible delayed, pregnancy was not inhibited. Future work will be directed to optimization of the vaccination schedule and alteration of the route of DNA administration in order to maximize antibody production prior to reproductive challenge.