REPRODUCTIVE PARAMETERS OF CHIMPANZEE MODEL

这项工作是针对调查雄激素依赖性 黑猩猩附睾内的蛋白质， 开发避孕疫苗。 我们已经确定EP 2为 雄激素依赖，并已确定了几个不同的剪接 EP 2基因的变体。 两个主要的变体，EP 2A和EP 2B， 不同之处在于它们由不同的启动子转录。 的 两种变体的N-末端序列都具有针对一种 信号肽酶切割位点，如分泌蛋白所预期。 使用EP 2的基因组克隆，我们正在鉴定 启动子和任何相关的雄激素结合序列。 我们 目前在细菌系统中表达EP 2蛋白， 在兔和鸡中产生抗体是不令人满意的， 以表达的蛋白为抗原。 当这种抗体 我们将继续研究，以阐明EP 2在 睾丸后精子成熟 作为这些研究的一个独立部分 我们发表了一份关于免疫组织化学的详细报告， 黑猩猩附睾分泌蛋白EP 1的定位 附睾

The work is directed to investigation of the androgen dependent proteins within the chimpanzee epididymis, with the potential for development of contraceptive vaccines. We have identified EP2 as androgen dependent and have identified several different splicing variants of the EP2 gene. The two main variants, EP2A and EP2B, differ in that they are transcribed by different promoters. The N-terminal sequence of both variants possess concensus sequences for a signal peptidase cleavage site, as expected for a secretory protein. Using a genomic clone of EP2, we are int the process of identifying the promoter and any associated androgen-binding sequences. We are currently expressing the EP2 protein in a bacterial system, as antibody generation in rabbits and chickens has been unsatisfactory, to use the expressed protein as antigen. When this antibody is obtained we will continue studies to elucidate the function of EP2 in post-testicular sperm maturation. As a separate part of these studies we have published a detailed report on the immunohistochemical localization of the epididymal secretory protein EP1 in the chimpanzee epididymis.