SUGARS AS NOVEL CRYOPROTECTANTS FOR PRIMATE OOCYTES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 辅助生殖，包括体外受精(IVF)，是自然受孕困难妇女医疗的重要组成部分。体外受精涉及对女性进行荷尔蒙刺激，以通过手术回收卵母细胞进行受精，以诱导多个卵泡发育。这一过程可能需要重复几次。冷冻保存雌配子将减少与重复试管受精相关的压力。虽然冷冻保存胚胎是可行的，但灵长类卵母细胞的冷冻保存还不是很成功。胚胎保存并不总是最好的选择，例如，在化疗前恢复卵母细胞等将允许在治疗时与伴侣无关的妇女未来使用试管受精。此外，与胚胎冷冻相关的某些法律问题也可以避免。卵母细胞的保存在濒危物种的保护计划中也是有价值的。这项工作在灵长类动物模型中测试了糖作为灵长类卵母细胞冷冻保护剂的使用，因为卵母细胞存在遗传差异，这排除了(例如)仓鼠卵母细胞储存方法的应用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Assisted reproduction, including in vitro fertilization (IVF) is an important part of medical treatment for women who have difficulty conceiving naturally. IVF involves hormonal stimulation of the woman to induce multiple follicle development with surgical recovery of oocytes for fertilization. This procedure may have to be repeated on several occasions. Freeze preservation of female gametes would reduce the stress associated with repeated IVF. While it is practical to freeze preserve embryos, freeze preservation of primate oocytes has not been predictable successful. Embryo storage is not always the best option, for example when oocyte recovery prior to chemotherapy etc. would allow future use of IVF for a woman not associated with a partner at the time of treatment. In addition, certain legal problems associated with embryo freezing could be avoided. Oocyte preservation would also be of value in conservation programs for endangered species. This work tests the use of sugars as cryoprotective agents for primate oocytes in the primate model as there are genetic differences in oocytes which have precluded application of methods developed for storage of (for example) hamster oocytes.