BEHAVIORAL & PHYSIOLOGICAL CONSEQUENCES OF LOSS

行为的

• 批准号: 6219676
• 负责人: JULIE M WORLEIN
• 金额: $ 12.74万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6219676
• 关键词: AIDS; Mammalia; animal tissue; behavioral /social science research tag; communicable diseases; hematology; immunology; inflammation; psychology; virus

It has been shown that separating nonhuman primates from their mothers for a short period of time as infants results in permanent alterations in the immune system. What has not been determined is whether immune changes due to this stressor result in increased risk for morbidity or mortality. In this study we are evaluating disease progression following inoculation with SIVe660 in 20 four-year-old pigtailed macaques (Macaca nemestrina). The subjects were part of an experimental protocol at the University of Colorado, where their behavioral and immune development was closely monitored since infancy. Half of the subjects experienced a two-week maternal separation at six months of age and half did not. To date seven monkeys have been shipped to Seattle for SIV inoculation. One subject has been infected for 28 weeks and four have been infected for 8 weeks. Two additional animals are currently undergoing quarantine and will be infected in March. Blood draws taken in Seatt le b efore infection showed that animals that undergwent separation had higher proportions of suppressor/cytotoxic T lympocytes (CD8+) and natural killer cells (CD16+) than controls. They also had higher hematocrit and hemoglobin levels. In the 8 weeks after infection proportions of CD8+ cells increased for all subjects while proportions of CD4+ (Helper T lymphocytes) and CD20+ (nonactivated B lymphocytes) decreased significantly. Subjects are being tested weekly on the amount of consumption of a preferred liquid (Kool Aid). All subjects showed a significant increase in consumption in the first 8 weeks of infection; there was no difference between animals that had experienced separation and those that had not in the amount of liquid consumed. Behavioral observations are also conducted three times per week to determine behavioral changes due to infection. FUNDING NIH grant RR00166 and NIMH grants MH37373, MH19514, MH45045, and MH15442.

研究表明，将非人类灵长类动物与它们的 母亲在婴儿时期短暂哺乳会导致永久性的 免疫系统的改变。 尚未确定的是 这种压力源导致的免疫变化是否会导致风险增加 发病率或死亡率。 在这项研究中，我们正在评估疾病 20 名四岁儿童接种 SIVe660 后病情进展 辫子猕猴（Macaca nemestrina）。 受试者是一个 科罗拉多大学的实验方案，他们的 从婴儿期起就密切监测行为和免疫发育。 一半的受试者在六岁时经历了两周的产妇分离 几个月大了一半没有。 迄今为止，已有七只猴子被 运往西雅图进行 SIV 接种。 一名受试者已被感染 已感染 28 周，其中 4 人已感染 8 周。 另外两个 动物目前正在接受检疫，将在 行进。 感染前在西雅图进行的抽血显示 经历分离的动物有更高的比例 抑制/细胞毒性 T 淋巴细胞 (CD8+) 和自然杀伤细胞 (CD16+) 比对照。 他们的血细胞比容和血红蛋白也较高 水平。 感染后8周CD8+细胞比例 所有受试者的 CD4+ 比例均有所增加（辅助 T 淋巴细胞）和 CD20+（未激活的 B 淋巴细胞）减少 显著地。 每周都会对受试者进行测试 饮用首选液体（Kool Aid）。 所有受试者均表现出 感染前8周内消耗量显着增加； 经历过的动物之间没有区别 分离和那些没有消耗的液体量。 每周还进行 3 次行为观察，以 确定由于感染引起的行为变化。 资助 NIH 补助金 RR00166 和 NIMH 授予 MH37373、MH19514、MH45045 和 MH15442。