BEHAVIORAL & PHYSIOLOGICAL CONSEQUENCES OF LOSS: SIV

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• 批准号: 6316795
• 负责人: JULIE M WORLEIN
• 金额: $ 13.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6316795
• 关键词: AIDS; Mammalia; animal tissue; behavioral /social science research tag; communicable diseases; hematology; immunology; inflammation; psychology; virus

It has been shown that separating nonhuman primates from their mothers for a short period of time as infants results in permanent alterations in the immune system. What has not been determined is whether immune changes due to this stressor result in increased risk for morbidity or mortality. In this study we are evaluating disease progression following inoculation with SIVe660 in 20 four-year-old pigtailed macaques (Macaca nemestrina). The subjects were part of an experimental protocol at the University of Colorado, where their behavioral and immune development was closely monitored since infancy. Half of the subjects experienced a two-week maternal separation at six months of age and half did not. To date seven monkeys have been shipped to Seattle for SIV inoculation. One subject has been infected for 28 weeks and four have been infected for 8 weeks. Two additional animals are currently undergoing quarantine and will be infected in March. Blood draws taken in Seatt le b efore infection showed that animals that undergwent separation had higher proportions of suppressor/cytotoxic T lympocytes (CD8+) and natural killer cells (CD16+) than controls. They also had higher hematocrit and hemoglobin levels. In the 8 weeks after infection proportions of CD8+ cells increased for all subjects while proportions of CD4+ (Helper T lymphocytes) and CD20+ (nonactivated B lymphocytes) decreased significantly. Subjects are being tested weekly on the amount of consumption of a preferred liquid (Kool Aid). All subjects showed a significant increase in consumption in the first 8 weeks of infection; there was no difference between animals that had experienced separation and those that had not in the amount of liquid consumed. Behavioral observations are also conducted three times per week to determine behavioral changes due to infection. FUNDING NIH grant RR00166 and NIMH grants MH37373, MH19514, MH45045, and MH15442.

研究表明，将非人类灵长类动物与它们的 母亲在婴儿时期的一段短时间内， 免疫系统的改变。 尚未确定的是 这种应激源引起的免疫变化是否会增加 发病率或死亡率。 在这项研究中，我们评估疾病 20名4岁儿童接种SIVe 660后的进展 长尾猕猴（Macaca nemestrina）。 受试者是一个 科罗拉多大学的实验方案， 从婴儿期开始就密切监测行为和免疫发育。 一半的受试者在六岁时经历了为期两周的母亲分离 半个月的年龄没有。 到目前为止，已经有七只猴子 运到西雅图进行SIV接种 一个实验对象被感染了 28周，4人感染8周。 两个附加 动物目前正在接受检疫， 三月 在感染前在Seatt le B抽取的血液显示， 经历分离的动物有更高比例的 抑制性/细胞毒性T淋巴细胞（CD 8+）和自然杀伤细胞 (CD16+）的对照组。 他们的红细胞压积和血红蛋白也更高 程度. 感染后8周，CD 8+细胞比例 所有受试者的CD 4+（辅助T细胞）比例均增加， 淋巴细胞）和CD 20+（非活化B淋巴细胞）减少 显著 受试者每周接受一次 最好的饮料（Kool Aid）。 所有受试者均表现出 在感染的前8周内，消费量显著增加; 在经历过同样的经历的动物之间 分离和那些没有在液体消耗量。 每周还进行三次行为观察， 确定感染引起的行为变化。 资助NIH赠款 RR 00166和NIMH赠款MH 37373、MH 19514、MH 45045和MH 15442。