CORE--NMR SPECTROSCOPY FACILITY

核磁共振波谱仪

• 批准号: 6396721
• 负责人: MICHAEL WEISS
• 金额: --
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6396721
• 关键词: biomedical facility; fluorescence spectrometry; nuclear magnetic resonance spectroscopy

The University of Chicago Cancer Research Center (UCCRC) Core Facility in Nuclear Magnetic Resonance (NMR) copy offers state-of-the-art instrumentation in multidimensional heteronuclear NMR spectroscopy and phase-modulation fluorescence spectroscopy for application to basic research in molecular oncology. These methods enable the structure and dynamics of cancer-related macromolecules (such as oncoproteins and growth factors) and drug interactions to be investigated in solution. The goals of the facility are: (a) to provide advice to faculty and trainees regarding the capabilities and appropriate scientific role of such methods in the context of their fields, (b) to provide "hands-on" teaching and training, (c) to provide 2D, 3D and 4D homonuclear and heteronuclear NMR spectra for analysis of the structures of proteins, carbohydrates and nucleic acids in solution, (d) to provide heteronuclear relaxation times at multiple field strengths for analysis of macromolecular dynamics, (e) to provide complementary steady-state and phase-modulation fluorescence spectra of macromolecules, and (f) to provide a workstation for computer-based data analysis. The NMR spectrometers were purchased in 1995 using funds from the Division of the Biological Sciences and peer-reviewed matching funds from the National Cancer Institute. The Facility is located in the newly constructed Biological Sciences Learning Center/Jules F. Knapp Medical Research Building. Scheduling information and data output from the instruments are accessible to faculty in their offices via high-speed computer data links.

芝加哥大学癌症研究中心（UCCRC）的核心设施， 核磁共振（NMR）副本提供最先进的 多维异核核磁共振光谱仪器和 相位调制荧光光谱法在基础研究中的应用 分子肿瘤学的研究。这些方法使结构和 癌症相关大分子（如癌蛋白和 生长因子）和药物相互作用。的 该设施的目标是：（a）向教员和受训人员提供咨询意见 关于这些机构的能力和适当的科学作用， （B）提供“实践” （c）提供2D、3D和4D的教学和培训， 用于分析蛋白质结构的杂波NMR光谱， 碳水化合物和核酸，（d）提供异源性 在多种场强下的弛豫时间，用于分析 大分子动力学，（e）提供互补的稳态和 大分子的相位调制荧光光谱，以及（f）至 为基于计算机的数据分析提供工作站。的NMR 1995年，使用联合国海洋事务和海洋法司的资金购买了100台光谱仪。 生物科学和同行评审的国家配套资金 癌症研究所.该设施位于新建的 生物科学学习中心/Jules F.克纳普医学研究 建设从仪器输出的调度信息和数据是 通过高速计算机数据， 链接.