CORE--NMR SPECTROSCOPY FACILITY

芝加哥大学癌症研究中心的核心设施在 核磁共振(核磁共振)复制品提供最先进的 多维异核核磁共振谱的仪器和 位相调制荧光光谱学在碱性条件下的应用 分子肿瘤学研究。这些方法使结构和 与癌症相关的大分子(如癌蛋白和 生长因子)和药物在溶液中的相互作用。这个 该设施的目标是：(A)向教职员工和受训人员提供咨询意见 关于这种生物的能力和适当的科学作用 方法在其领域的上下文中，(B)提供“实践” 教学和培训，(C)提供2D、3D和4D同核和 用于分析蛋白质结构的异核核磁共振波谱， 溶液中的碳水化合物和核酸，(D)提供异核 用于分析多个场强的弛豫时间 大分子动力学，(E)提供补充的稳态和 大分子的位相调制荧光光谱，以及(F)至 为基于计算机的数据分析提供一个工作站。核磁共振 光谱仪是在1995年购买的，资金来自 生物科学和同行评议的国家配套基金 癌症研究所。该设施位于新建的 生物科学学习中心/Jules F.Knapp医学研究 大楼。从仪器输出的调度信息和数据是 教职员工可在其办公室通过高速计算机数据访问 链接。

The University of Chicago Cancer Research Center (UCCRC) Core Facility in Nuclear Magnetic Resonance (NMR) copy offers state-of-the-art instrumentation in multidimensional heteronuclear NMR spectroscopy and phase-modulation fluorescence spectroscopy for application to basic research in molecular oncology. These methods enable the structure and dynamics of cancer-related macromolecules (such as oncoproteins and growth factors) and drug interactions to be investigated in solution. The goals of the facility are: (a) to provide advice to faculty and trainees regarding the capabilities and appropriate scientific role of such methods in the context of their fields, (b) to provide "hands-on" teaching and training, (c) to provide 2D, 3D and 4D homonuclear and heteronuclear NMR spectra for analysis of the structures of proteins, carbohydrates and nucleic acids in solution, (d) to provide heteronuclear relaxation times at multiple field strengths for analysis of macromolecular dynamics, (e) to provide complementary steady-state and phase-modulation fluorescence spectra of macromolecules, and (f) to provide a workstation for computer-based data analysis. The NMR spectrometers were purchased in 1995 using funds from the Division of the Biological Sciences and peer-reviewed matching funds from the National Cancer Institute. The Facility is located in the newly constructed Biological Sciences Learning Center/Jules F. Knapp Medical Research Building. Scheduling information and data output from the instruments are accessible to faculty in their offices via high-speed computer data links.