CORE--CENTRALIZED HISTOPATHOLOGY LABORATORY

核心——集中组织病理学实验室

• 批准号: 6314530
• 负责人: ISAIAH J FIDLER
• 金额: $ 33.23万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314530
• 关键词: biomedical facility; histopathology; human tissue; immunocytochemistry; in situ hybridization; neoplastic cell; stainings; tissue /cell preparation

The goal of the Centralized Histopathology Laboratory (CHPL) is to provide histologic services to investigators at M.D. Anderson Cancer Center. The CHPL supplies technical support and consultation, develops or applies appropriate techniques, and maintains the consistency and high quality needed to perform these techniques. The CHPL is currently located in the Department of Pathology in 375 feet/2. Because of the increasing emphasis on in vivo systems in cancer biology and developmental biology, the number of laboratories using tissue specimens, transgenic mice, and gene-knockout technologies has increased dramatically. To provide efficient histology services to the institution, the CHPL will be expanded, and a second 370- feet/2 laboratory will be located in the R.E. "Bob" Smith Research Building. The well-equipped and well-staffed CHPL processes fixed and frozen specimens for routine histologic analysis, immunohistochemical in situ hybridization (colorimetric procedures), and special staining for tissue sections and cells. The CHPL (both sites) is directed by Dr. Isaiah J. Fidler, and is accessible to all faculty, at M.D. Anderson Cancer Center, will preference given to investigators with peer-reviewed grants. Among the services provided are rapid preparation of high-quality standard histologic sections; application of special technologies as the need arises for selected projects, including preparation of frozen sections and histochemical, immunohistochemical, and tissue sections for in situ hybridization analysis; consultation with investigators to tailor technology to their special needs; and interpretation of histologic data. To access the CHPL, investigators make a formal application. After approval by the Director, the histotechnologist meets with the investigators to determine special needs of the study and the technical staff provides the histopathological services. The services are prioritized and coordinated to provide timely service. The development of new technologies that require additional equipment or special supplies has been funded by M.D. for specialized procedures such as in situ hybridization and 500 specially stained slides yearly. The increase in studies of transgenic mice and gene knockout mice will significantly increase the CHPL's workload, especially for special preparations such as serial sections of whole-embryos mounts. The CHPL is an essential component of the M.D. Anderson centralized research facilities that require histologic analysis.

中央组织学实验室（CHPL）的目标是提供 为医学博士的研究者提供组织学服务。安德森癌症中心。的 CHPL提供技术支持和咨询，开发或应用 适当的技术，并保持一致性和高质量 需要使用这些技术。CHPL目前位于 病理科在375英尺/2。由于越来越强调 在癌症生物学和发育生物学的体内系统上， 使用组织标本、转基因小鼠和基因敲除 技术已经急剧增长。为了提供有效的组织学 服务的机构，CHPL将扩大，第二个370- 英尺/2的实验室将位于R.E.“鲍勃”史密斯研究 建设设备齐全、人员配备齐全的CHPL流程固定， 冷冻标本用于常规组织学分析，免疫组化， 原位杂交（比色法）和特殊染色， 组织切片和细胞。CHPL（两个站点）由以赛亚博士指导 J. Fidler，并可访问所有教师，在医学博士。安德森癌症 中心，将优先考虑研究人员与同行评审的赠款。 提供的服务包括快速准备高质量标准的 组织切片;根据需要应用特殊技术 包括制作冻结切片， 组织化学、免疫组织化学和组织切片原位 杂交分析;与研究者协商， 技术，以满足他们的特殊需要;和解释组织学数据。 为了访问CHPL，调查人员提出正式申请。后 经主任批准，组织技术专家与 研究者确定研究的特殊需求和技术 工作人员提供组织病理学服务。这些服务 优先考虑并协调提供及时的服务。的发展 需要额外设备或特殊供应的新技术 由医学博士资助。对于专门的程序，如在现场 杂交和每年500张特殊染色的载玻片。的增加 转基因小鼠和基因敲除小鼠的研究将显著 增加CHPL的工作量，特别是特殊准备工作，例如 整个胚胎的连续切片。CHPL是一个重要的 M.D.的组成部分。安德森集中研究设施， 需要组织学分析