CORE--CENTRALIZED HISTOPATHOLOGY LABORATORY

中央组织病理学实验室(CHPL)的目标是提供 向M.D.安德森癌症中心的研究人员提供组织学服务。这个 CHPL提供技术支持和咨询，开发或应用 适当的技术，并保持一致性和高质量 需要执行这些技术。社区公共图书馆目前位于 病理学系在375英尺/2。因为越来越强调 癌症生物学和发育生物学中的体内系统，数量 使用组织样本、转基因小鼠和基因敲除的实验室 技术已经大幅增加。提供高效的组织学 向该机构提供的服务，CHPL将扩大，第二个370- 英尺/2实验室将设在R.E.《鲍勃·史密斯研究》 大楼。设备齐全、人员齐全的CHPL流程固定和 冰冻标本用于常规组织学分析，免疫组织化学 原位杂交(比色法)和特殊染色 组织切片和细胞。CHPL(两个站点)由以赛亚博士指导 J.Fidler，并对M.D.安德森癌症学院的所有教职员工开放 中心，将优先考虑获得同行审查拨款的调查人员。 所提供的服务包括快速编制高质量的标准 组织切片.必要时特殊技术的应用 产生于选定的项目，包括制作冰冻切片和 原位组织化学、免疫组织化学和组织切片 杂交分析；咨询调查人员以量身定做 技术以满足他们的特殊需求；以及组织学数据的解释。 要进入CHPL，调查人员需要提出正式申请。之后 经署长批准，组织技术员将与 调查人员要确定研究的特殊需要和技术 工作人员提供组织病理学服务。这些服务包括 优先安排和协调，提供及时的服务。的发展。 需要额外设备或特殊用品的新技术 由医学博士资助进行专门的手术，如原位手术 杂交和每年500张特殊染色的幻灯片。这一增长 转基因小鼠和基因敲除小鼠的研究将具有重要意义 增加CHPL的工作量，特别是特殊准备工作，如 连续的全胚胎切片。CHPL是一个必不可少的 M.D.安德森中心研究设施的组成部分 需要进行组织学分析。

The goal of the Centralized Histopathology Laboratory (CHPL) is to provide histologic services to investigators at M.D. Anderson Cancer Center. The CHPL supplies technical support and consultation, develops or applies appropriate techniques, and maintains the consistency and high quality needed to perform these techniques. The CHPL is currently located in the Department of Pathology in 375 feet/2. Because of the increasing emphasis on in vivo systems in cancer biology and developmental biology, the number of laboratories using tissue specimens, transgenic mice, and gene-knockout technologies has increased dramatically. To provide efficient histology services to the institution, the CHPL will be expanded, and a second 370- feet/2 laboratory will be located in the R.E. "Bob" Smith Research Building. The well-equipped and well-staffed CHPL processes fixed and frozen specimens for routine histologic analysis, immunohistochemical in situ hybridization (colorimetric procedures), and special staining for tissue sections and cells. The CHPL (both sites) is directed by Dr. Isaiah J. Fidler, and is accessible to all faculty, at M.D. Anderson Cancer Center, will preference given to investigators with peer-reviewed grants. Among the services provided are rapid preparation of high-quality standard histologic sections; application of special technologies as the need arises for selected projects, including preparation of frozen sections and histochemical, immunohistochemical, and tissue sections for in situ hybridization analysis; consultation with investigators to tailor technology to their special needs; and interpretation of histologic data. To access the CHPL, investigators make a formal application. After approval by the Director, the histotechnologist meets with the investigators to determine special needs of the study and the technical staff provides the histopathological services. The services are prioritized and coordinated to provide timely service. The development of new technologies that require additional equipment or special supplies has been funded by M.D. for specialized procedures such as in situ hybridization and 500 specially stained slides yearly. The increase in studies of transgenic mice and gene knockout mice will significantly increase the CHPL's workload, especially for special preparations such as serial sections of whole-embryos mounts. The CHPL is an essential component of the M.D. Anderson centralized research facilities that require histologic analysis.