MATRILYSIN IN LUNG EPITHELIAL CELL INJURY AND REPAIR

基质溶素在肺上皮细胞损伤和修复中的作用

基本信息

  • 批准号:
    6202222
  • 负责人:
  • 金额:
    $ 20.75万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1999
  • 资助国家:
    美国
  • 起止时间:
    1999-09-01 至 2000-08-31
  • 项目状态:
    已结题

项目摘要

We have observed the strong expression of the MMP matrilysin by alveolar epithelial cells following alveolar injury associated with organizing diffuse alveolar damage, post-bone marrow transplant idiopathic pneumonia syndrome, and interstitial pneumonitis. Matrilysin is not found in the respiratory epithelium of normal lung. Following lung surgery, matrilysin was detected by immunohistochemistry and in situ hybridization in "row" of type II cells marching across denuded alveolar walls. Matrilysin-positive cells were found largely in areas of damaged or destroyed basement membrane and rested on a fibronectin-containing matrix. Matrilysin is frequently an "epithelial cell-derived" MMP, but other MMPs which are known to be produced by some epithelial cells, namely collagenase-I, stromelysin-1, and gelatinase B (MMP-9), were not produced by human lung respiratory epithelium. In vitro studies of both rat and human alveolar epithelial cells demonstrated strong capacity for matrilysin mRNA and protein expression. Expression of matrilysin was also markedly induced in airway epithelial cells in cystic fibrosis and severe asthma, again suggesting a function in injury-mediated responses. Supporting this concept, we found enhanced matrilysin expression by migrating human airway (tracheal) epithelial cells in an ex vivo model of tissue repair. In such explants, following mechanical wounding, immunoreactive matrilysin was present both within and underneath the migrating tracheal epithelial cells. Based upon these observations, we hypothesize that matrilysin expression by type II pneumocytes accompanies severe alveolar injury, and speculate that the proteolytic activity of this MMP is needed to facilitate cell migration and repair. To address this hypothesis, we propose the following specific aims: 1) we will examine the effect of matrilysin expression by alveolar epithelial cells on alveolar repair following experimental acute lung injury; 2) we will determine the effects of matrilysin on human and rodent alveolar epithelial cell behavior in vitro; and 3) we will define how matrilysin expression is regulated in human and rodent alveolar epithelial cells. To accomplish these objectives, we will employ diverse cell biological and molecular biologic approaches including the study of matrilysin-deficient versus wild-type mice. We will determine whether matrilysin is necessary for the migration of type II pneumocytes, whether contact of cells with provisional matrix proteins stimulates matrilysin expression, and the intracellular mechanisms of matrilysin induction in alveolar epithelial cells.
我们观察到肺泡中MMP基质溶解素的强烈表达

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

HOWARD G WELGUS其他文献

HOWARD G WELGUS的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('HOWARD G WELGUS', 18)}}的其他基金

MATRILYSIN IN LUNG EPITHELIAL CELL INJURY AND REPAIR
基质溶素在肺上皮细胞损伤和修复中的作用
  • 批准号:
    6505082
  • 财政年份:
    2001
  • 资助金额:
    $ 20.75万
  • 项目类别:
MATRILYSIN IN LUNG EPITHELIAL CELL INJURY AND REPAIR
基质溶素在肺上皮细胞损伤和修复中的作用
  • 批准号:
    6347589
  • 财政年份:
    2000
  • 资助金额:
    $ 20.75万
  • 项目类别:
MATRILYSIN IN LUNG EPITHELIAL CELL INJURY AND REPAIR
基质溶素在肺上皮细胞损伤和修复中的作用
  • 批准号:
    6109689
  • 财政年份:
    1998
  • 资助金额:
    $ 20.75万
  • 项目类别:
EXPRESSION OF MATRIX METALLOPROTEINASES BY MONOCYTES AND ROLE IN LUNG BIOLOGY
单核细胞表达基质金属蛋白酶及其在肺生物学中的作用
  • 批准号:
    6241789
  • 财政年份:
    1997
  • 资助金额:
    $ 20.75万
  • 项目类别:
MATRIX METALLOPROTEINASES IN CUTANEOUS WOUND HEALING
基质金属蛋白酶在皮肤伤口愈合中的作用
  • 批准号:
    2079106
  • 财政年份:
    1984
  • 资助金额:
    $ 20.75万
  • 项目类别:
MATRIX METALLOPROTEINASES IN CUTANEOUS WOUND HEALING
基质金属蛋白酶在皮肤伤口愈合中的作用
  • 批准号:
    2079105
  • 财政年份:
    1984
  • 资助金额:
    $ 20.75万
  • 项目类别:
MATRIX METALLOPROTEINASES IN CUTANEOUS WOUND HEALING
基质金属蛋白酶在皮肤伤口愈合中的作用
  • 批准号:
    2769562
  • 财政年份:
    1984
  • 资助金额:
    $ 20.75万
  • 项目类别:
MATRIX METALLOPROTEINASES IN CUTANEOUS WOUND HEALING
基质金属蛋白酶在皮肤伤口愈合中的作用
  • 批准号:
    2517434
  • 财政年份:
    1984
  • 资助金额:
    $ 20.75万
  • 项目类别:
THE BIOLOGICAL ROLE OF HUMAN SKIN COLLAGENASE INHIBITOR
人类皮肤胶原酶抑制剂的生物学作用
  • 批准号:
    3157366
  • 财政年份:
    1984
  • 资助金额:
    $ 20.75万
  • 项目类别:
THE BIOLOGICAL ROLE OF HUMAN SKIN COLLANGENASE INHIBITOR
人类皮肤胶原酶抑制剂的生物学作用
  • 批准号:
    3071281
  • 财政年份:
    1984
  • 资助金额:
    $ 20.75万
  • 项目类别:

相似海外基金

RUI: Mechanoregulation of Collective Cell Migration in Biomimetic Microenvironments
RUI:仿生微环境中集体细胞迁移的机械调节
  • 批准号:
    2342274
  • 财政年份:
    2024
  • 资助金额:
    $ 20.75万
  • 项目类别:
    Standard Grant
Uncovering the Underlying Biophysical Mechanisms of Directed Cell Migration
揭示定向细胞迁移的潜在生物物理机制
  • 批准号:
    2345411
  • 财政年份:
    2024
  • 资助金额:
    $ 20.75万
  • 项目类别:
    Standard Grant
CAREER: Predictive Multiscale Modeling of Cell Migration through Pores between Endothelial Cells
职业:通过内皮细胞之间的孔进行细胞迁移的预测多尺度建模
  • 批准号:
    2339054
  • 财政年份:
    2024
  • 资助金额:
    $ 20.75万
  • 项目类别:
    Standard Grant
Collaborative Research: DMS/NIGMS 1: Simulating cell migration with a multi-scale 3D model fed by intracellular tension sensing measurements
合作研究:DMS/NIGMS 1:使用由细胞内张力传感测量提供的多尺度 3D 模型模拟细胞迁移
  • 批准号:
    2347957
  • 财政年份:
    2024
  • 资助金额:
    $ 20.75万
  • 项目类别:
    Standard Grant
Collaborative Research: DMS/NIGMS 1: Simulating cell migration with a multi-scale 3D model fed by intracellular tension sensing measurements
合作研究:DMS/NIGMS 1:使用由细胞内张力传感测量提供的多尺度 3D 模型模拟细胞迁移
  • 批准号:
    2347956
  • 财政年份:
    2024
  • 资助金额:
    $ 20.75万
  • 项目类别:
    Standard Grant
Mitochondrial positioning regulates redox-signaling during cell migration
线粒体定位调节细胞迁移过程中的氧化还原信号
  • 批准号:
    10520211
  • 财政年份:
    2023
  • 资助金额:
    $ 20.75万
  • 项目类别:
Actin gating of crosstalk between Rho GTPases in cell migration
细胞迁移中 Rho GTP 酶之间串扰的肌动蛋白门控
  • 批准号:
    10736927
  • 财政年份:
    2023
  • 资助金额:
    $ 20.75万
  • 项目类别:
Full Project 1: Defining Mechanisms of MICAL-dependent Pancreatic Cancer Cell Migration
完整项目 1:MICAL 依赖性胰腺癌细胞迁移的定义机制
  • 批准号:
    10762273
  • 财政年份:
    2023
  • 资助金额:
    $ 20.75万
  • 项目类别:
Localized mitochondrial metabolic activity in Xenopus mesendoderm cells undergoing collective cell migration
爪蟾中内胚层细胞集体细胞迁移的局部线粒体代谢活性
  • 批准号:
    10751722
  • 财政年份:
    2023
  • 资助金额:
    $ 20.75万
  • 项目类别:
Mechanisms underlying a decline in neural stem cell migration during aging
衰老过程中神经干细胞迁移下降的机制
  • 批准号:
    10750482
  • 财政年份:
    2023
  • 资助金额:
    $ 20.75万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了