UNUSUAL POST SPECIES EVOLUTION OF MHC CLASS I LOCI IN NEW WORLD PRIMATES

新世界灵长类动物 MHC I 类基因座的异常后物种进化

• 批准号: 6116456
• 负责人: David I Watkins
• 金额: $ 7.6万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116456
• 关键词: Mammalia; animal tissue; immunology; inborn metabolism disorder; inflammation; technology /technique; transplantation

OBJECTIVE To determine the nature of the MHC class I loci in New World Primates. RESULTS Homologues of the human major histocompatibility complex (MHC) HLA-A, -B, -E, -F, and -G loci are present in all the Catarrhini (Old World primates, apes, and humans), and some of their allelic lineages have survived several speciation events. Analysis of 26 MHC class I cDNAs from seven different genera of New World primates revealed that the Callitrichinae (tamarins and marmosets) are an exception to these rules of MHC stability. In gene trees of primate MHC class I genes, sequences from the Callitrichinae cluster in a genus-specific fashion, whereas in the other genera of New World primates, as in the Catarrhini, they cluster in a transgeneric way. DISCUSSION The genus-specific clustering of the Callitrichinae cDNAs indicates that there is no orthology between MHC class I loci in genera of this phyletic group. Additionally, the Callitrichinae genera exhibit limited variability of their MHC class I genes, in contrast to the high variability displayed by all other primates. Each Callitrichinae genus, therefore, expresses its own set of MHC class I genes, suggesting that an unusually high rate of turnover of loci occurs in this subfamily. The limited variability of MHC class I genes in the Callitrichinae is likely the result of the recent origin of these loci. FUTURE DIRECTIONS These data will facilitate an understanding of the immunogenetics of New World Primates. Several of these species are important animal models for human diseases (i.e. ulcerative colitis and multiple sclerosis). KEY WORDS MHC, 1D IEF, PBL FUNDING NIH RR00167, DK44886 PUBLICATIONS Cadavid, LF, Shufflebotham, C, Ruiz, FJ, Yeager, M, Hughes, AL, and D.I. Watkins. 1998. Unusual post-species evolution of the MHC class I loci in New World primates. Proc. Natl. Acad. Sci., USA 94:14536-14541.

确定新的MHC I级基因座的性质 世界灵长类动物。 人类专业的结果同源物 组织相容性复合物（MHC）HLA -A，-b，-e，-f和-g基因座是 出现在所有卡塔尔尼（旧世界的灵长类动物，猿和人类）中 他们的一些等位基因谱系在几个物种物种中得以幸存 事件。 分析来自七个不同属的26个MHC I类cDNA 新世界灵长类动物揭示了Callitrichinae（tamarins和 marmosets）是这些MHC稳定性规则的例外。 在基因 灵长类动物MHC I类基因的树，Callitrichinae的序列 以特定属的方式簇，而在另一个属中 新世界灵长类动物，例如在卡塔尔尼（Catarrhini）中，它们聚集在一个 转变方式。 讨论属的特定于属的聚类 Callitrichinae cDNA表示MHC之间没有矫正 该植物组的属I级基因座。 另外， Callitrichinae属表现出其MHC I类的有限可变性 基因，与所有其他所有其他显示的高变异性相反 灵长类动物。 因此，每个Callitrichinae属都表达了自己的集合 MHC I类基因的 基因座的营业额发生在此亚家族中。 有限的可变性 Callitrichinae中的MHC I类基因可能是 这些基因座的最新起源。 未来的方向这些数据将 有助于理解新世界的免疫遗传学 灵长类动物。 这些物种中有几个是重要的动物模型 人类疾病（即溃疡性结肠炎和多发性硬化症）。 钥匙 单词MHC，1D IEF，PBL资助NIH RR00167，DK44886出版物 Cadavid，LF，Shufflebotham，C，Ruiz，FJ，Yeager，M，Hughes，Al和Al和Al D.I.沃特金斯。 1998年。MHC类的不寻常的后种类演变 我在新世界灵长类动物中所在。 Proc。 纳特。 学院。 美国科学 94：14536-14541。