IV TRANSMISSION OF SIV RT VIRIONS

SIV RT 病毒粒子的 IV 传播

• 批准号: 6116699
• 负责人: CHRISTOPHER J MILLER
• 金额: $ 7.98万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116699
• 关键词: AIDS; Mammalia; clinical research; communicable diseases; immunology; inflammation; intestines; lymphatic system; reproductive system; sexually transmitted diseases; vaccines; virus; women's health

448 Significance HIV is primarily a sexually transmitted disease but little is known about the transmission of viral variants during sexual contact. An understanding of the types of viruses transmitted by sex may make it possible to design vaccines to prevent the transmission of HIV. It has long been held that the genetic material in the virion was RNA only, but it has been shown recently that some transcription of RNA to DNA occurs normally in both SIV and HIV virions. Further is has been demonstrated that virions with DNA transcripts are more efficient at infecting resting lymphocytes than virions without these transcripts. Objectives In this study, we used the rhesus macaque/SIV model to test the 2 hypothesis. One is that reverse transcribed (RT) viruses replicate more efficiently than normal virions. The second is that reverse transcribed (RT) viruses are more efficiently transmitted by sexual contact. Results Eight animals were inoculated intravenously with normal virions and 8 were inoculated with RT-virions. Plasma virus loads during the first eight weeks of infection are currently being assessed. Six animals were inoculated intravaginally with a low dose of SIVmac251 RT, while six animals were inoculated with the same amount of normal virions. Thus far none of the animals have become infected with either of these virus stocks. Thus the presence of DNA transcripts does not seem to have a marked effect on the efficiency at which a virus is transmitted across the vaginal mucosa. Future Directions Studies are continuing in order to determine if there is a subtle effect on transmission that was not noted in this study. KEY WORDS viral variants, HIV mucosal transmission FUNDING NIH Grants AI35545 and RR00169

448意义艾滋病毒主要是一种性传播疾病 但人们对病毒变异体的传播知之甚少， 性接触 了解传播的病毒类型 可以设计疫苗来预防 艾滋病毒的传播。 长期以来，人们一直认为遗传物质 在病毒体中只有RNA，但最近的研究表明， SIV和HIV都正常地将RNA转录为DNA 病毒体。 此外，已经证明，具有DNA的病毒体 转录本感染静息淋巴细胞的效率比 没有这些转录本的病毒体。 目的在本研究中，我们使用 恒河猴/SIV模型来检验2个假设。 一是 逆转录（RT）病毒的复制效率高于 正常的病毒体 第二种是逆转录病毒 更容易通过性接触传播 结果八 用正常病毒体静脉内接种动物， 接种RT病毒体。 前八个月的血浆病毒量 目前正在评估感染情况。 六只动物 阴道内接种低剂量的SIVmac 251 RT，而6 用相同量的正常病毒体接种动物。 因此 迄今为止，没有任何动物感染过这两种病毒 病毒库存。 因此，DNA转录本的存在似乎并不 对病毒传播的效率有显著影响 穿过阴道粘膜 未来方向研究仍在继续 为了确定是否存在对传输的微妙影响， 在这项研究中没有注意到。 关键词病毒变异，HIV粘膜 NIH赠款AI 35545和RR 00169