CHOLERA TOXIN AS AN ADJUVANT FOR GENITAL SIV IMMUNITY
CHOLERA TOXIN AS AN ADJUVANT FOR GENITAL SIV IMMUNITY
批准号:
6116693
负责人:
CHRISTOPHER J MILLER
金额:
$7.98万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-05-01 至 2000-04-30
中文摘要
HIV是一种性传播疾病,也是一种疫苗
英文摘要
Significance HIV is a sexually transmitted disease and a vaccine
capable of preventing sexual transmission of HIV should elicit mucosal
immune responses in the genital tract. Objectives This study should
determine if immunization at distant mucosal sites can result in
vaginal anti-SIV immune responses and whether the mucosa adjuvant
cholera toxin (CT) was useful for this purpose. Results Two rhesus
macaques were orally immunized with CT mixed with 1000ug of SIVp55 and
2 animals were orally immunized with CT mixed with 250ug of SIVp55.
The animals immunized with CT and high dose antigen made strong
systemic immune responses to P55 including antibody, T cell
proliferative and T cell cytokine responses to P55. The immune
responses in the animal immunized with low dose P55 were weak.
Significant levels of anti-p55 IgA antibodies were found in the rectal
secretions but not in the vaginal secretions of these animals. Four
rhesus macaques were intranasally immunized with CT mixed with SIVp55
and 2 animals were intranasally immunized with 10ug CT mixed with
250ug of SIVp55. The animals immunized with CT made strong systemic
immune responses to P55 including antibody, T cell proliferative and T
cell cytokine responses to P55. Significant levels of anti-p55 IgA
antibodies were found in the rectal secretions and in the vaginal
secretions of these animals. This stud y demonstrates that the
mucosal adjuvant CT is effective in rhesus macaques. In addition, it
is now clear that oral immunization is not an effective method for
generating mucosal immune responses in the vagina and that intranasal
immunization is an excellent route of immunization for eliciting
vaginal immunity. Future Directions Test other vaccine preparations
by intranasal route. KEY WORDS mucosal immunity, intranasal
immunization, SIV antigens FUNDING NIH Grants AI35545 and RR00169
PUBLICATIONS Kubota, M. C.J. Miller, K. Imaoka, S. Kawabata, K.
Fujihashi, J.R. McGhee, H. Kiyono. Oral immunization with simian
immunodeficiency virus (SIV) p55 gag and cholera toxin (CT) elicits
both mucosal IgA and systemic IgG immune responses in non-human
primates. J. Immunol. 158 5321-5329. 1997. Imaoka, K., C.J.
Miller, M. Kubota, S. Kawabata, M.B. McChesney, K. Someya, M.
Yamamoto, K. Fujihashi, M. Honda, J.R. McGhee, H. Kiyono. Nasal
immunization of non-human primates with simian immunodeficiency virus
(SIV) p55 gag and cholera toxin (CT) adjuvant induces Th1/Th2 help for
virus specific immune responses in reproductive tissues. J. Immunol.
161:5952-5958, 1998. Kawabata, S., C.J. Miller, T. Lehner, K.
Fujihashi, M. Kubota, J.R. McGhee, T. Hiroi, H. Kiyono. Induction of
Th2 cytokine expression for p27-specific IgA B cell responses after
TLN immunization with SIV antigens in rhesus macaques. J. Infect.
Dis. 177:26-33, 1998.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
EFFECT OF STEROID HORMONES ON GENITAL IMMUNITY
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批准号:6430505
-
项目类别:
-
资助金额:$16.56万
-
财政年份:2001
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
EFFECT OF STEROID HORMONES ON GENITAL IMMUNITY
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批准号:6577261
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项目类别:
-
资助金额:$16.56万
-
财政年份:2001
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
EFFECT OF STEROID HORMONES ON GENITAL IMMUNITY
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批准号:6314114
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项目类别:
-
资助金额:$16.49万
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财政年份:2000
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负责人:CHRISTOPHER J MILLER
-
依托单位:
EFFECT OF STEROID HORMONES ON GENITAL IMMUNITY
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批准号:6108652
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项目类别:
-
资助金额:$16.49万
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财政年份:1999
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
IV TRANSMISSION OF SIV RT VIRIONS
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批准号:6116699
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项目类别:
-
资助金额:$7.98万
-
财政年份:1999
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES
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批准号:6116698
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项目类别:
-
资助金额:$7.98万
-
财政年份:1999
-
负责人:CHRISTOPHER J MILLER
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依托单位:
CELLULAR PATHWAY OF VAGINAL SIV INFECTION
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批准号:6116694
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项目类别:
-
资助金额:$7.98万
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财政年份:1999
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
SIV CHALLENGE OF SHIV INOCULATED RHESUS MACAQUES
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批准号:6116700
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项目类别:
-
资助金额:$7.98万
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财政年份:1999
-
负责人:CHRISTOPHER J MILLER
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依托单位:
VAGINAL MICROBICIDES TO PREVENT VAGINAL SIV TRANSMISSION
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批准号:6116697
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项目类别:
-
资助金额:$7.98万
-
财政年份:1999
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
EFFECT OF STEROID HORMONES ON GENITAL IMMUNITY
-
批准号:6116695
-
项目类别:
-
资助金额:$8.99万
-
财政年份:1999
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
ENHANCEMENT OF IMMUNE RESPONSES TO P55
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批准号:6116696
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项目类别:
-
资助金额:$8.99万
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财政年份:1999
-
负责人:CHRISTOPHER J MILLER
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依托单位:
COMPARISON OF INTRAVENOUS VERSUS MUCOSAL INOCULATION W/ SIV
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批准号:6277935
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项目类别:
-
资助金额:$8.13万
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财政年份:1998
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负责人:CHRISTOPHER J MILLER
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依托单位:
VAGINAL TRANSMISSION OF SIV RT VIRIONS
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批准号:6277941
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项目类别:
-
资助金额:$8.13万
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财政年份:1998
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负责人:CHRISTOPHER J MILLER
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依托单位:
TITRATION OF SIV BY VAGINAL INOCULATION
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批准号:6277938
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项目类别:
-
资助金额:$8.13万
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财政年份:1998
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负责人:CHRISTOPHER J MILLER
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依托单位:
CHARACTERIZATION OF PATHOGENIC SHIVS
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批准号:6277931
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项目类别:
-
资助金额:$8.13万
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财政年份:1998
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负责人:CHRISTOPHER J MILLER
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依托单位:
MUCOSAL IMMUNIZATION AGAINST GENITAL TRANSMISSION OF SIV
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批准号:6277936
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项目类别:
-
资助金额:$8.13万
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财政年份:1998
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负责人:CHRISTOPHER J MILLER
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依托单位:
VAGINAL IMMUNOLOGY IN MENSTRUAL CYCLES
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批准号:6277940
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项目类别:
-
资助金额:$8.13万
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财政年份:1998
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
TRANSIENT VIREMIA FOLLOWING LOW DOSE INTRAVAGINAL INOCULATION OF SIV
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批准号:6277939
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项目类别:
-
资助金额:$8.13万
-
财政年份:1998
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
EFFECT OF STEROID HORMONES ON GENITAL IMMUNITY
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批准号:6272250
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项目类别:
-
资助金额:$16.26万
-
财政年份:1998
-
负责人:CHRISTOPHER J MILLER
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依托单位:
SIV CHALLENGE OF SHIV INOCULATED RHESUS MACAQUES
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批准号:6277937
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项目类别:
-
资助金额:$8.13万
-
财政年份:1998
-
负责人:CHRISTOPHER J MILLER
-
依托单位:
海外基金