RECOMBINANT PICORNAVIRUSES AS AIDS VACCINES

重组小核糖核酸病毒作为艾滋病疫苗

• 批准号: 6116698
• 负责人: CHRISTOPHER J MILLER
• 金额: $ 7.98万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116698
• 关键词: AIDS; Mammalia; clinical research; communicable diseases; immunology; inflammation; lymphatic system; microorganism; reproductive system; sexually transmitted diseases; virus; women's health

Significance HIV is a sexually transmitted disease and a vaccine capable of preventing sexual transmission of HIV should elicit mucosal immune responses in the genital tract. Objectives To determine recombinant polioviruses expressing SIV antigens can elicit immune responses. Results Five animals were immunized intranasally (IN) with recombinant polioviruses. All animals made anti-SIV IgA responses in rectal secretions and 4 of 5 had systemic anti-SIV antibodies. Two of 5 animals had detectable anti-SIV IgG and IgA antibodies in vaginal secretions. Future Directions Dr. Andino is improving the recombinant polio vectors to increase stability and expression of the inserted genes. Once these new vectors are available, we will test their immunogenicity in macaques. If robust immunity is observed with these new polio vectors, we will challenge the immunized animals intravaginally with SIV. KEY WORDS vaccine protection, mucosal immunity, SIV vaginal transmission FUNDING NIH Grants AI36178 and RR00169

意义艾滋病毒是一种性传播疾病和疫苗 能够防止性传播艾滋病毒的人应该引起粘膜 生殖道中的免疫反应。确定目标 表达SIV抗原的重组脊髓灰质炎病毒可诱导免疫 回应。结果5只动物鼻腔免疫5只，免疫效果良好。 重组脊髓灰质炎病毒。所有动物均呈抗SIV-IgA反应 直肠分泌物和全身抗SIV抗体阳性4例。其中两个 5只动物阴道内可检测到抗SIV抗体 分泌物。未来方向安迪诺博士正在改进重组 脊髓灰质炎载体增加插入的稳定性和表达 基因。一旦这些新的载体可用，我们将测试他们的 猕猴的免疫原性。如果观察到这些强健的免疫力 新的脊髓灰质炎载体，我们将挑战免疫动物 阴道内注射SIV。关键词疫苗保护，粘膜 免疫，SIV阴道传播基金NIH赠款AI36178和 RR00169