PRIMATE MODEL OF MENOPAUSAL HOT FLASHES

目的测定皮肤温度和电导率 (衡量出汗的电学指标)可以用恒河猴来测量 猕猴用于发展这一动物模型的研究 潮热的病因学。结果实验动物未被麻醉。 电极和热敏电阻，用于测量皮肤电导和 温度分别施加于耳廓、额头、 雌性恒河猴的小腿和胸部处于束缚状态 这只动物以前习惯了很久的椅子 句号。这只动物被允许随意获得食物和水， 并在数据记录期间被连续观看 精确控制温度和湿度的房间。数据是 每两天录制一次，每天三到五个小时。在第一次 一天后，进行90分钟的基线测量 室内温度在30分钟内起伏不定。 然后在35℃下保持约30分钟。皮肤温度 在基线期间是稳定的，然后显示预期的 对环境温度变化的生理反应。在.期间 在30分钟的加热期内，皮肤电导水平波动 在小腿上可以看到出汗的迹象。在……第二天 数据记录，使用相同的动物。基线测量结果为 获得125分钟，之后环境温度 缓慢上升到40℃。在基线期间，皮肤温度稳定， 在此之后，它们随着环境温度的升高而增加。皮肤电导 在胸骨和小腿上可以看到提示出汗的水平 采暖期。这些数据证明了该方案的可行性。 建议的录音程序。我们计划进行的未来方向 对去卵巢雌性猕猴进行的三项简短的先导研究 来年确定和验证一个或多个目标 指示热的发生的生理标志物 闪电侠。关键词：更年期、潮热、皮温、皮肤 电导；动物模型发展。资助NIH PO1 AG11915，R37 AG05233，R21申请待定。

OBJECTIVE To determine whether skin temperature and conductance (an electrical measure of sweating) could be measured in rhesus macaques for development of this animal model in the study of the etiology of hot flashes. RESULTS The animal was not anesthetized. Electrodes and thermisters, for measuring skin conductance and temperature, respectively, were applied to the ear pinna, forehead, calf and chest of a female rhesus macaque positioned in a restraining chair to which the animal had been previously accustomed for prolonged periods. The animal was allowed ad libitum access to food and water, and was watched continuously during data recording which took place in a room under precise temperature and humidity control. Data were recorded on two days for three to five hours each day. On the first day, baseline measurements were obtained for 90 minutes after which the room temperature raised and lowered a few degrees for 30 minutes then held at 35{C for approximately 30 minutes. Skin temperatures were stable during the baseline period and then showed the expected physiological responses to the ambient temperature changes. During the 30 minute heating period, fluctuations in skin conductance level sugges tive of sweating were seen in the calf. On the second day of data recording, the same animal was used. Baseline measurements were obtained for 125 minutes after which the ambient temperature was slowly raised to 40{C. Skin temperatures were stable during baseline, after which they increased with ambient temperature. Skin conductance level suggestive of sweating was seen in the sternum and calf during the heating period. The data demonstrate the feasibility of the proposed recording procedures. FUTURE DIRECTIONS We plan to conduct three short pilot studies in ovariectomized female macaques in the coming year to identify and validate one or more objective physiological markers that would indicate the occurance of a hot flash. KEY WORDS menopause, hot flashes, skin temperature; skin conductance; animal model development. FUNDING NIH PO1 AG11915, R37 AG05233, R21 application pending.