NEUROBIOLOGY SENSORY GATING

这个项目的目的是分离神经生物学的 脑移植中感觉门控的机制 将死胎的组织送入人的前房 老鼠主人的眼睛。组织将在例行程序中获得 堕胎，适当的大脑区域将被解剖，而 移植物将植入眼球的前房。 裸鼠裸鼠。在那里，胎儿组织会变大， 分化，从而形成神经元。这些人类神经元 然后可以用标准的神经生物学技术进行研究 通常为动物模型保留，包括细胞外和 细胞内单个神经元记录，药理学与特异性 激动剂和拮抗剂，体内电化学，以及各种 组织学程序。一些组织将会被获取 为患有精神分裂症的妇女堕胎；与 项目2，将进行联系研究，以评估 该组织可能携带有增加风险的基因。 治疗精神分裂症。我们的目标是确定具体的 神经生物学缺陷，这将解释在 在这些特殊的标本中可以发现感觉门。 最初的研究将集中在海马体上，但 对于项目3和4，我们将集中在显示的领域 最有可能成为神经生物学底物的候选者 感官门控。利用眼内移植我们可以研究 或者，通过移植两个区域，我们可以 在两个不同的大脑之间产生突触连接 地区。

The aim of this project is to isolate the neurobiological mechanism responsible for sensory gating in transplants of brain tissue from dead human fetuses into the anterior chamber of the eye of rat hosts. The tissue will be obtained during routine abortions, appropriate brain areas will be dissected, and the graft will be placed into the anterior chamber of the eye of an athymic nude rat. There the fetal tissue increases in size and differentiates so that neurons are formed. These human neurons can then be studied with standard neurobiological techniques usually reserved for animal models, including extracellular and intracellular single neuron recording, pharmacology with specific agonists and antagonists, in vivo electrochemistry, and a variety of histological procedures. Some of the tissue will be obtained from abortions of women with schizophrenia; in co-operation with project 2, linkage studies will be performed to assess whether the tissue is likely to carry genes which convey increased risk for schizophrenia. We aim to determine if specific neurobiological deficits which would account for the deficit in sensory gating can be found in those particular specimens. Initial studies will be focused on the hippocampus, but, working with projects 3 and 4, we will concentrate on areas shown to be the most likely candidates for the neurobiological substrate of sensory gating. Using the in oculo transplants we can study single brain areas or, by transplanting two areas, we can generate synaptic connections between two different brain regions.