IN VITRO CULTURE OF RHESUS MONKEY FOLLICLES VIABILITY & MATURATION OF OOCYTE

对从卵巢中取出的卵母细胞进行细胞学评估。 未培养和培养的恒河猴卵泡，包括生殖器 囊泡染色质组织、减数分裂和蛋白质模式 使用荧光显微镜的磷酸化 从500 μ m以上的有腔卵泡中收集的CEOs， 和月经周期的晚期卵泡期的影响， 收集时间、培养时间和不同培养基对减数分裂的影响 观察到明显更多的卵母细胞提取自 卵泡在月经周期的早期卵泡阶段 进展过去核仁染色质的无框阶段比从 后期卵母细胞培养后，这种差异消失 减数分裂的完成随着培养时间的增加而增加。 表皮生长因子对卵母细胞培养液的刺激作用 有减数分裂能力的细胞和完成减数分裂的细胞 新鲜分离的腔前卵泡（<250 m）卵母细胞， 在培养中不能恢复减数分裂卵泡培养4 - 8 天显着增加了过去的卵母细胞的百分比 然而，在无边缘阶段，没有一个完成减数分裂

A cytological evaluation is being made of oocytes retrieved from uncultured and cultured rhesus follicles This includes germinal vesicle chromatin organization, meiosis, and patterns of protein phosphorylation using fluorescence microscopy Cumulus enclosed oocytes (CEOs) from antral follicles over 500 m were collected in the early and late follicular phase of the menstrual cycle The effect of the time of collection, hours in culture and different media on meiotic competence were observed Significantly more oocytes extracted from follicles during the early follicular phase of the menstrual cycle progressed past the unrimmed stage of nucleolar chromatin than from the late phase After oocyte culture, this difference disappeared Completion of meiosis increased with hours in culture The addition of epidermal growth factor to oocyte culture medium increased the percent that became meiotically competent and those that completed meiosis Freshly isolated oocytes from preantral follicles (<250 m) were incapable of resuming meiosis in culture Follicle culture from 4 to 8 days significantly increased the percent of oocytes progressing past the unrimmed stage None, however, completed meiosis