OOCYTE CHROMATIN ORGANIZATION IN FOLLICULOGENESIS & OOGENESIS IN M MULATTA

卵泡发生中的卵细胞染色质组织

• 批准号: 6591329
• 负责人: LORNA D JOHNSON
• 金额: $ 11.11万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6591329
• 关键词: Mammalia; biotechnology; clinical research; genetic manipulation; genetically modified animals; reproductive system; women's health

Follicles were collected from ovaries removed under halothane anaesthesia from 7 rhesus monkeys Follicle and ovum diameters were measured and chromatin organization was determined by fluorescence microscopy The relative growth curve of oocytes and follicles using a loess regression technique displayed a triphasic curve The relationship of follicle and oocyte growth and oocyte maturation (perinucleolar chromatin configuration) was analyzed Chromatin organization progressed in relation to ovum size In terms of follicle size, oocytes first respond (past the unrimmed nucleolar stage) during the period of earliest antrum formation, then the response remains relatively the same until the follicle reaches 600 m when the majority of responders are at the partial rim stage But it is not until the follicle has reached a diameter beyond 1000 m that the majority of oocytes are at the fully rimmed stage (meiotically competent)

从氟烷下取出的卵巢中收集卵泡 7只恒河猴的卵泡和卵子直径 染色质的组织用荧光法测定 显微镜观察卵母细胞和卵泡的相对生长曲线， 黄土回归曲线呈三相性。 卵泡和卵母细胞生长与卵母细胞成熟的关系 （核仁周围染色质构型）进行了分析染色质 组织化的进展与卵子的大小有关 大小，卵母细胞首先响应（过去的无缘核仁阶段）， 最早期的胃窦形成，然后反应仍然存在 相对相同，直到毛囊达到600米时，大多数 的响应者处于部分边缘阶段，但直到 卵泡直径超过1000米， 卵母细胞处于完全边缘阶段（减数分裂能力）