IN VITRO CULTURE OF RHESUS MONKEY FOLLICLES VIABILITY & MATURATION OF OOCYTE

正在对从中国提取的卵母细胞进行细胞学评估 未培养和培养的恒河猴卵泡，包括生发 囊泡染色质组织、减数分裂和蛋白质模式 用荧光显微镜观察卵丘封闭卵母细胞的磷酸化 (CEO)从500米以上的有腔卵泡中收集的早期 和卵泡晚期对月经周期的影响 采集时间、培养时间和不同培养基对减数分裂的影响 观察到有能力的人从 月经周期早期卵泡期的卵泡 经过核仁染色质的无框阶段，而不是从 在卵母细胞培养的后期，这种差异消失了 随着培养时间的增加，减数分裂的完成率增加。 表皮生长因子对卵母细胞培养液的促进作用 达到减数分裂能力的和完成减数分裂的 从腔前卵泡(&lt；250m)新鲜分离的卵母细胞 培养卵泡培养4~8天不能恢复减数分裂 天数显著增加了卵母细胞进展通过的百分比。 然而，无框阶段None完成了减数分裂。

A cytological evaluation is being made of oocytes retrieved from uncultured and cultured rhesus follicles This includes germinal vesicle chromatin organization, meiosis, and patterns of protein phosphorylation using fluorescence microscopy Cumulus enclosed oocytes (CEOs) from antral follicles over 500 m were collected in the early and late follicular phase of the menstrual cycle The effect of the time of collection, hours in culture and different media on meiotic competence were observed Significantly more oocytes extracted from follicles during the early follicular phase of the menstrual cycle progressed past the unrimmed stage of nucleolar chromatin than from the late phase After oocyte culture, this difference disappeared Completion of meiosis increased with hours in culture The addition of epidermal growth factor to oocyte culture medium increased the percent that became meiotically competent and those that completed meiosis Freshly isolated oocytes from preantral follicles (<250 m) were incapable of resuming meiosis in culture Follicle culture from 4 to 8 days significantly increased the percent of oocytes progressing past the unrimmed stage None, however, completed meiosis