IN VITRO CULTURE OF PREANTRAL RHESUS FOLLICLES VIABILITY & MATURATION OF OOCYTE

腔前恒河猴卵泡活力的体外培养

• 批准号: 6277754
• 负责人: LORNA D JOHNSON
• 金额: $ 7.6万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277754
• 关键词: Mammalia; Primates; growth /development; reproductive system

A cytological evaluation is being made of oocytes retrieved from uncultured and cultured rhesus follicles. This includes germinal vesicle chromatin organization, meiosis, and patterns of protein phosphorylation using fluorescence microscopy. Cumulus enclosed oocytes (CEOs) from antral follicles over 500 m were collected in the early and late follicular phase of the menstrual cycle. The effect of the time of collection, hours in culture and different media on meiotic competence were observed. Significantly more oocytes extracted from follicles during the early follicular phase of the menstrual cycle progressed past the unrimmed stage of nucleolar chromatin than from the late phase. After oocyte culture, this difference disappeared. Completion of meiosis increased with hours in culture. The addition of epidermal growth factor to oocyte culture medium increased the percent that became meiotically competent and those that completed meiosis. Freshly isolated oocytes from preantral follicles (<250 m) were incapable of resuming meiosis in culture. Follicle culture from 4 to 8 days significantly increased the percent of oocytes progressing past the unrimmed stage. None, however, completed meiosis.

正在对从中国提取的卵母细胞进行细胞学评估 未培养和培养的恒河猴卵泡。这包括生发 囊泡染色质组织、减数分裂和蛋白质模式 荧光显微镜下的磷酸化。封闭积云 收集来自500米以上有腔卵泡的卵母细胞。 月经周期的早期和晚期卵泡期。的影响 收集时间、培养时间和不同的媒体 观察其减数分裂能力。更多的卵母细胞 从早期卵泡期的卵泡中提取 月经周期超过了核仁的无框阶段 染色质比来自晚期的更多。在卵母细胞培养之后，这个 分歧消失了。减数分裂完成率随着时间的推移而增加 文化。在卵母细胞培养中添加表皮生长因子 Medium增加了变得具有减数分裂能力的百分比 完成减数分裂的那些。新鲜分离的腔前卵母细胞 卵泡(&lt；250m)在培养过程中不能恢复减数分裂。 卵泡培养4~8d可显著提高卵泡发育率。 经过无边框阶段的卵母细胞。然而，没有一个人， 减数分裂完成。