EFFECT OF INULIN CONSUMPTION ON VARIOUS ASPECTS OF HUMAN PHYSIOLOGY

菊粉消耗对人体生理各个方面的影响

• 批准号: 6117080
• 负责人: JOANNE LOUISE SLAVIN
• 金额: $ 4.48万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-01 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117080
• 关键词: apolipoproteins; biological products; blood lipid; blood lipoprotein; cholesterol; clinical research; clinical trials; dietary calcium; dietary iron; dietary supplements; enteric bacteria; feces analysis; fermentation; gastrointestinal nutrient absorption; glucose tolerance; glucose tolerance test; human subject; insulin sensitivity /resistance; inulin; magnesium; male; nutrition related tag; triglycerides; urinalysis; vegetables

This study will measure the effects of short-term consumption of inulin on blood lipid concentrations, iron, magnesium, and calcium absorption, fermentation in the colon, and glucose tolerance and insulin response. Inulin is isolated from chicory and is a naturally occuring storage oligomer of the carbohydrate fructose found in many plants, such as onion, garlic, artichoke, and chicory. To determine the physiological effects of a commercially-available inulin, twelve male subjects, ages 35-50 years, will consume controlled diets containing either no inulin or 15 grams of inulin per day. Subjects will complete daily questionnaires on their subjective responses to the diet. At the end of each 3 week period, the following measurements will be completed: blood lipids, including serum cholesterol, HDL-cholesterol, LDL-cholesterol, serum triglycerides, Apo A1, and Apo B. During the third week of each 3 week period, 5 day composites of feces and urine will be collected. In these samples, calcium, magnesium and iron absorption will be measured by determining fecal and urinary losses. From the 5 day fecal collection, fecal wet weight and dry weight, fecal pH, transit time, fecal short chain fatty acids, fecal bile acids and neutral steroids, an fecal enzymes will be measured. A final fresh fecal collection will be used for measurement of bacterial flora including total anaerobes, bifidobacteria, lactobacilli, enterococci, bacteroides, clostridia and coliforms (IMVIC series). Finally, glucose tolerance tests and insulin response will be measured in during the third week of each diet.

这项研究将测量短期食用菊粉对血脂浓度，铁，镁和钙吸收，结肠发酵，葡萄糖耐量和胰岛素反应的影响。菊糖是从菊苣中分离出来的，是一种天然存在的碳水化合物果糖的低聚物，存在于许多植物中，如洋葱、大蒜、朝鲜蓟和菊苣。 为了确定市售菊粉的生理作用，12名年龄在35-50岁的男性受试者将每天食用不含菊粉或含15克菊粉的受控饮食。 受试者将完成关于他们对饮食的主观反应的每日问卷。 在每3周结束时，将完成以下测量：血脂，包括血清胆固醇、HDL-胆固醇、LDL-胆固醇、血清甘油三酯、Apo A1和Apo B。在每3周的第3周期间，将收集5天粪便和尿液的混合物。 在这些样本中，将通过测定粪便和尿液损失来测量钙、镁和铁的吸收。 从5天粪便采集开始，将测量粪便湿重和干重、粪便pH值、通过时间、粪便短链脂肪酸、粪便胆汁酸和中性类固醇，并测量粪便酶。 最终新鲜粪便采集将用于测量细菌植物群，包括总厌氧菌、双歧杆菌、乳酸杆菌、肠球菌、类杆菌、梭菌和大肠菌群（IMVIC系列）。 最后，将在每种饮食的第三周期间测量葡萄糖耐量试验和胰岛素反应。