STRUCTURE OF MUSCLE THICK FILAMENT: MYOSIN AND PARAMYOSIN

肌肉粗丝的结构：肌球蛋白和副肌球蛋白

• 批准号: 6120568
• 负责人: HENRY F. EPSTEIN
• 金额: --
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120568
• 关键词: bacteria; biomedical resource; growth /development

They are interested. in the structure of the ACDS (Acetyl-CoA Decarbonylase SYnd'ase) multienzyme complex which is responsible for the synthesis and cleavage of acetyl-CoA in methanogens. To determine the detailed oligorneric structure of the complex ( -1.6-2 MDA), accurate molecular masses are needed. ACDS complexes from Methanosarcina therinophila and M. barkeri contain five different subunits designated -a, P, y, 6, and F- The complex can also be dissociated into three protein subcomponents, one of which is (a + e). It is thought that the (a+e) particle is a heterotetramer (oc2e2) of approximately 220 kDa, but gel filtration data have indicated a value of 350 kDa. To start this project, fi-eeze-dried specimens of the (a+c:) particle were prepared. Preliminary ma s measurements indicate that it is a heterotetramer. The (y + 8) subcomponent from the complex has also been examined in the STEM for mass analysis, which is being performed now.

他们很感兴趣。 在ACDS（乙酰辅酶A）的结构中， 脱羰酶（脱羧酶）多酶复合物，其负责 产甲烷菌中乙酰辅酶A的合成和裂解。 以确定 复合物的详细寡聚体结构（-1.6-2 MDA）， 需要精确的分子量。 ACDS复合物， 嗜热甲烷八叠球菌（Methanosarcina therinophila）和M. barkeri包含五种不同的 亚基命名为-a、P、y、6和F。 解离成三种蛋白质亚组分，其中之一是（a + e）。 认为（a+e）颗粒是以下的异四聚体（α 2 e2）： 约220 kDa，但凝胶过滤数据表明， 350 kDa。 为了开始这个项目， 制备（a+c：）颗粒。 初步的火星测量表明 它是异源四聚体。 （y + 8）子分量 复杂的也已经在STEM中进行了质量分析， 现在正在执行。