COMPARATIVE BACKBONE DYNAMICS OF NTRC IN ITS ACTIVE & INACTIVE CONFORMATIONS

NTRC 活动状态下的骨干动力学比较

• 批准号: 6121011
• 负责人: BRIAN F VOLKMAN
• 金额: $ 1.54万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121011
• 关键词: biological products; biomedical resource; enzymes; human tissue; neoplasm /cancer; nuclear magnetic resonance spectroscopy; proteins

The major goal of this project is to: Determine the metabolic capabilities of the human uroepithelial cells, for activation of 4-aminobiphenyl and its N-hydroxy metabolites. Identify which of the proximate metabolites are converted to reactive electrophilic species. Identify the DNA-adducts that are generated by interaction of the ultimate electrophile with the DNA. Evaluate the biological effects of interaction of 4-aminobiphenyl derivatives with urothelial DNA - at the level of (A) mutation and (B) neoplastic transformation.

该项目的主要目标是：确定代谢 人类泌尿上皮细胞的能力，用于激活 4-氨基联苯及其N-羟基代谢物。 识别哪个 近似代谢物被转化为活性亲电子物质。 鉴定由DNA与DNA的相互作用产生的DNA加合物。 DNA的终极亲电体 评估生物效应 4-氨基联苯衍生物与尿路上皮DNA的相互作用 （A）突变水平和（B）肿瘤转化水平。