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SALT DEPENDENCE OF PROCESSIVITY OF T45G RIBONUCLEASE

SALT DEPENDENCE OF PROCESSIVITY OF T45G RIBONUCLEASE
T45G 核糖核酸酶持续性的盐依赖性
批准号:
6298200
负责人:
RONALD T RAINES
金额:
$0.75万
依托单位国家:
美国
项目类别:
财政年份:
1999
资助国家:
美国
项目状态:
已结题
起止时间:
1999-03-01 至 2000-02-29

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中文摘要
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英文摘要
The salt dependence of processivity of the Threonine 45 to Glycine (T45G) mutation of Ribonuclease A will be determined using a 31P NMR based assay for processivity of T45G acting on Polyadenosine (PolyA) [S.B. delCardayr R.T. Raines, Biochemistry 33, 6031-6037 (1994)]. The NMR assay allows for the direct determination of the processivity parameter, PI, of T45G at a variety of salt conditions. From the changes in PI with the changes in salt concentration, one can assess the importance of the coulombic interactions between the positively charged basic residues on T45G with the negatively charged phosphates on PolyA on processivity in this system.
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ENERGETICS OF CATALYSIS BY RIBONUCLEASES
  • 批准号:
    6309205
  • 项目类别:
  • 资助金额:
    $0.75万
  • 财政年份:
    2000
  • 负责人:
    RONALD T RAINES
  • 依托单位:
SALT DEPENDENCE OF PROCESSIVITY OF T45G RIBONUCLEASE
  • 批准号:
    6309203
  • 项目类别:
  • 资助金额:
    $0.75万
  • 财政年份:
    2000
  • 负责人:
    RONALD T RAINES
  • 依托单位:
CYS32 PKA MEASUREMENTS OF MUTANTS THIOREDOXINS W/ ALTERED REDOX POTENTIALS
  • 批准号:
    6309204
  • 项目类别:
  • 资助金额:
    $0.75万
  • 财政年份:
    2000
  • 负责人:
    RONALD T RAINES
  • 依托单位:
SMALL MOLECULE CATALYST OF PROTEIN FOLDING
  • 批准号:
    6309206
  • 项目类别:
  • 资助金额:
    $0.75万
  • 财政年份:
    2000
  • 负责人:
    RONALD T RAINES
  • 依托单位:
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