TRANSCRIPTIONAL REGULATION OF WT-1

WT-1 的转录调控

• 批准号: 6236706
• 负责人: GRADY F SAUNDERS
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6236706
• 关键词: DNA footprinting; RNA splicing; RNase protection assay; Wilms' tumor; gel mobility shift assay; gene expression; genetic enhancer element; genetic library; genetic mapping; genetic models; genetic promoter element; genetic regulation; genetic regulatory element; genetic transcription; human genetic material tag; in situ hybridization; molecular cloning; neoplasm /cancer genetics; nucleic acid probes; nucleic acid sequence; reporter genes; transcription factor; transposon /insertion element

The goal of this project is to understand the major elements involved in transcriptional regulation of the Wilms' tumor WT1 gene. Experiments are designed to identify and clone novel trans-acting proteins (enhancers, silencer, promoter elements) involved in the modulation of WT1 expression. The genetic loci for these transacting regulatory proteins will be determined since mutation at these loci may be important in the development of Wilms' tumor.

这个项目的目标是了解参与的主要因素， Wilms肿瘤WT 1基因的转录调控。实验 设计用于鉴定和克隆新的反式作用蛋白（增强子， 沉默子、启动子元件）。 这些反式调节蛋白的遗传基因座将是 因为这些基因座上的突变可能在 Wilms肿瘤的发展