SALIVARY PROTEINS AND ORAL BACTERIAL ADHESION

唾液蛋白质和口腔细菌粘附

• 批准号: 6238379
• 负责人: DONALD I HAY
• 金额: $ 25.79万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 1998-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6238379
• 关键词: cell adhesion; cell adhesion molecules; host organism interaction; human subject; hydroxyapatites; laboratory rabbit; oral bacteria; pathologic process; saliva; tooth enamel

Our past studies in model systems, in vitro, have shown that specific salivary proteins, adsorbed onto synthetic hydroxyapatite (HA), selectively promote adhesion of several prominent oral bacterial species to HA. These findings strongly suggest that specific salivary proteins, adsorbed onto teeth, may play an important role in the highly selective initial adhesion of oral bacteria that occurs naturally on tooth surfaces. It is now important to test this hypothesis, in vivo. To make this test, and to advance knowledge in this area, the following aims are proposed. AIM 1 To test the central hypothesis that specific salivary proteins, adsorbed onto dental enamel surfaces, promote selective bacterial adhesion to those surfaces, in vivo. Pure samples of selected salivary proteins, known to promote adhesion of prominent oral bacteria to HA in vitro, will be used to treat enamel blocks. The blocks will be mounted on a prosthetic device, and the device placed in the mouth. The initial adhesion of selected bacteria to the protein-treated enamel will be determined over several hours in eight subjects by combined cultural and DNA checkerboard analyses using 16S rRNA probes. The microbial adhesion data will be analyzed statistically for relationships with the protein pretreatments. AIM 2 j To test the hypothesis that a subject's salivary protein profile determines pellicle composition, and that this influences selective bacterial adhesion to dental enamel in vivo. Salivary composition is poorly defined. Therefore, adsorption of specific bacterial adhesion-promoting proteins onto HA will be quantified, in vitro, for the eight subjects used in Aim 1, using techniques developed for that purpose. Thus, the amounts of the different adhesion-promoting proteins adsorbing onto the mineral will be determined. Each subject's microbial adhesion data, obtained in Aim 1, will be analyzed statistically for relationships with the same subject's salivary protein profile and protein adsorption data. AIM 3 To test the hypothesis that some salivary and serum proteins and bacterial macromolecules such as glucans and glycosyl-transferases act as inhibitors of adhesion of specific oral bacteria to HA-adsorbed salivary proteins. The possible role of some salivary and serum proteins and selected bacterial products as inhibitors of initial microbial adhesion is a further important activity that must be systematically quantified to understand selective microbial adhesion to teeth, in vivo. Therefore, the effects of chromatographic fractions from saliva and serum, and the noted bacterial macromolecules, on specific bacteria-salivary protein adhesion reactions will be determined, in vitro, As appropriate, these data will be incorporated into the interpretation of results of Aim 1.

我们过去在体外模型系统中的研究表明， 唾液蛋白，吸附在合成羟基磷灰石（HA）上， 选择性促进几种主要口腔细菌的粘附 到HA。 这些发现有力地表明，特定的唾液蛋白， 吸附在牙齿上，可能在高度选择性的 口腔细菌在牙齿上自然发生初始粘附 表面。 现在重要的是在体内检验这一假设。 使 这项测试，并在这方面的知识，以下目标是 提出了 目的1验证特异性唾液腺分泌的中心假说， 蛋白质，吸附在牙釉质表面，促进选择性 细菌粘附到这些表面，在体内。 所选的纯样品 唾液蛋白，已知可促进主要口腔细菌的粘附 HA体外，将用于治疗釉质块。 这些积木将 安装在假肢装置上，并将该装置放置在口腔中。 的 所选细菌对蛋白质处理的釉质的初始粘附将 在八个科目中，通过结合文化因素， 和使用16S rRNA探针的DNA棋盘格分析。 微生物 将对粘附数据进行统计学分析，以确定与 蛋白质预处理。 目的2 j为了检验受试者的 唾液蛋白质谱决定表膜组成，这 影响细菌对牙釉质的选择性粘附。 唾液成分定义不明确。 因此，吸附 HA上的特异性细菌粘附促进蛋白将 对于目标1中使用的8名受试者，使用 为此开发的技术。 因此， 不同的粘附促进蛋白吸附到矿物质上， 测定 目标1中获得的每例受试者的微生物粘附数据， 将对同一受试者的关系进行统计分析 唾液蛋白质谱和蛋白质吸附数据。 目的3：测试 一些唾液和血清蛋白质和细菌 大分子如葡聚糖和糖基转移酶作为 特定口腔细菌对HA吸附唾液的粘附抑制剂 proteins. 一些唾液和血清蛋白的可能作用， 作为初始微生物粘附抑制剂的选定细菌产物 是另一项重要活动，必须系统地量化 来了解在体内微生物对牙齿的选择性粘附。 因此，我们认为， 来自唾液和血清的色谱组分的影响，以及 注意到细菌大分子，在特定的细菌唾液蛋白 将在体外测定粘附反应，如适用，这些 数据将被纳入目标1结果的解释中。