SALIVARY PROTEINS AND BACTERIAL ADHESION TO ORAL SURFACE

唾液蛋白质和口腔表面的细菌粘附

• 批准号: 3222398
• 负责人: DONALD I HAY
• 金额: $ 21.76万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3222398
• 关键词: Actinomyces; Streptococcus mutans; Streptococcus sanguis; cell adhesion; chemical structure function; dental plaque; disease /disorder proneness /risk; epithelium; hydroxyapatites; laboratory mouse; laboratory rabbit; membrane activity; monoclonal antibody; oligosaccharides; oral bacteria; oral mucosa; oral pharyngeal; pellicle; protein engineering; proteins; proteolysis; saliva

Dental caries and various forms of periodontal disease are etiologically related to the accumulation of certain types of bacteria on teeth, in the form of dental plaques. An understanding of plaque formation is, therefore, of considerable significance. This application focuses on a primary step in oral microbial colonization, i.e., the role of specific salivary proteins in promoting, or impairing adhesion of prominent oral bacteria to tooth and soft tissue surfaces. Three aims are proposed. Aim I is a microbiological and biochemical study aimed at (a) identifying specific salivary proteins which bind to hydroxyapaptite (HA) and selectively mediate adhesion of strains of Streptococcus mutans, S. sanguis, S. mitior, Actinomyces viscosus and A. naeslundii, (b) identifying salivary proteins which may bind to bacterial cells and prevent their adhesion to HA, and (c) identifying salivary proteins which modulate adhesion of bacteria to human buccal epithelial cells. Aim II is an immunological, biochemical and microbiological study aimed at the systematic identification and quantitation of salivary proteins, and peptides from their degradation, which provide specific binding sites for bacterial adhesion to teeth or which otherwise modulate bacterial adhesion. Poly- and monoclonal antibodies specific for relevant proteins and peptides will be made and used to (a) develop immunoassays to identify and measure moieties present in saliva and dental pellicle, (b) establish relationships between the presence and concentrations of a given salivary protein(s) or peptide(s) and adhesion of an oral microorganism. Aim III is a biochemical and microbiological study aimed at (a) elucidating the molecular basis of the adhesion modulating properties of proteins identified in Aim I, by sequencing, by study of defined molecular segments obtained by specific proteolysis, and by determining the effect of specific chemical modifications on adhesion modulating activity, (b) investigating the use of synthetic and natural peptide and oligosaccharide analogs to extend structure-activity studies, and (c) determining the effects of modifying HA surfaces using molecular segments from known HA-reactive proteins, and synthetic peptides on microbial adhesion. Results from these multidisciplinary studies are essential for understanding the initial step in the formation of dental plaque, and they should provide a basis for developing new methods for its prevention and control.

龋齿和各种形式的牙周病是 在病因学上与某些类型的 牙齿上的细菌，以牙菌斑的形式。 的理解 因此，牙菌斑的形成具有相当重要的意义。 该应用程序集中在口腔微生物的主要步骤 殖民化，即，特定唾液蛋白的作用 促进或损害主要口腔细菌粘附于 牙齿和软组织表面。 提出了三个目标。 目标I是 微生物和生物化学研究，旨在（a）确定 结合羟基磷灰石（HA）的特异性唾液蛋白， 选择性介导变形链球菌菌株的粘附， S. sanguis，S. mitior、粘性放线菌（Actinomycesviscosus）和A.内氏酵母，（B） 鉴定可与细菌细胞结合的唾液蛋白， 防止它们粘附到HA上，和（c）鉴定唾液蛋白 其调节细菌对人颊上皮的粘附 细胞 Aim II是一种免疫学、生物化学和 微生物研究旨在系统鉴定和 唾液蛋白质的定量，以及来自其 降解，为细菌提供特异性结合位点， 粘附到牙齿或以其它方式调节细菌粘附。 对相关蛋白质具有特异性的多克隆抗体和单克隆抗体， 肽将被制备并用于（a）开发免疫测定， 鉴定和测量唾液和牙齿中存在部分 表膜，（B）建立存在和 给定唾液蛋白或肽的浓度，和 口腔微生物的粘附。 目标III是一种生物化学和 微生物研究，目的是（a）阐明分子基础 的粘附调节特性的蛋白质鉴定， 目的I，通过测序，通过研究定义的分子片段 通过特异性蛋白水解获得，并通过测定 对粘附调节活性的特定化学修饰， (b)研究合成和天然肽的使用， 寡糖类似物，以扩展结构-活性研究，以及 (c)确定使用以下物质修饰HA表面的效果： 来自已知HA反应性蛋白的分子片段，和 合成肽对微生物粘附的影响。 从这些 多学科研究对于理解最初的 在牙菌斑形成的一步，他们应该提供一个 为开发新的预防和控制方法奠定了基础。