SALIVARY PROTEINS AND BACTERIAL ADHESION TO ORAL SURFACE

唾液蛋白质和口腔表面的细菌粘附

• 批准号: 3222397
• 负责人: DONALD I HAY
• 金额: $ 23.12万
• 依托单位: ADA FORSYTH INSTITUTE, INC.
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3222397
• 关键词: Actinomyces; Streptococcus mutans; Streptococcus sanguis; cell adhesion; chemical structure function; dental plaque; disease /disorder proneness /risk; epithelium; hydroxyapatites; laboratory mouse; laboratory rabbit; membrane activity; monoclonal antibody; oligosaccharides; oral bacteria; oral mucosa; oral pharyngeal; protein engineering; proteins; proteolysis; saliva

Dental caries and various forms of periodontal disease are etiologically related to the accumulation of certain types of bacteria on teeth, in the form of dental plaques. An understanding of plaque formation is, therefore, of considerable significance. This application focuses on a primary step in oral microbial colonization, i.e., the role of specific salivary proteins in promoting, or impairing adhesion of prominent oral bacteria to tooth and soft tissue surfaces. Three aims are proposed. Aim I is a microbiological and biochemical study aimed at (a) identifying specific salivary proteins which bind to hydroxyapaptite (HA) and selectively mediate adhesion of strains of Streptococcus mutans, S. sanguis, S. mitior, Actinomyces viscosus and A. naeslundii, (b) identifying salivary proteins which may bind to bacterial cells and prevent their adhesion to HA, and (c) identifying salivary proteins which modulate adhesion of bacteria to human buccal epithelial cells. Aim II is an immunological, biochemical and microbiological study aimed at the systematic identification and quantitation of salivary proteins, and peptides from their degradation, which provide specific binding sites for bacterial adhesion to teeth or which otherwise modulate bacterial adhesion. Poly- and monoclonal antibodies specific for relevant proteins and peptides will be made and used to (a) develop immunoassays to identify and measure moieties present in saliva and dental pellicle, (b) establish relationships between the presence and concentrations of a given salivary protein(s) or peptide(s) and adhesion of an oral microorganism. Aim III is a biochemical and microbiological study aimed at (a) elucidating the molecular basis of the adhesion modulating properties of proteins identified in Aim I, by sequencing, by study of defined molecular segments obtained by specific proteolysis, and by determining the effect of specific chemical modifications on adhesion modulating activity, (b) investigating the use of synthetic and natural peptide and oligosaccharide analogs to extend structure-activity studies, and (c) determining the effects of modifying HA surfaces using molecular segments from known HA-reactive proteins, and synthetic peptides on microbial adhesion. Results from these multidisciplinary studies are essential for understanding the initial step in the formation of dental plaque, and they should provide a basis for developing new methods for its prevention and control.

龋齿和各种形式的牙周病是 在病因学上与某些类型的 牙齿上的细菌，以牙菌斑的形式存在。一种理解 因此，斑块形成的研究具有相当重要的意义。 这一应用主要集中在口腔微生物的第一步 定植，即特定唾液蛋白在 促进或损害口腔主要细菌对 牙齿和软组织表面。提出了三个目标。Aim I是 一项微生物学和生化研究，目的是(A)确定 与羟基磷灰石(HA)结合的特定唾液蛋白和 选择性地介导变形链球菌菌株的黏附， S.sanguis、S.mior、粘性放线菌和A.nesnudii，(B) 识别可能与细菌细胞结合的唾液蛋白和 防止它们与透明质酸黏附，以及(C)鉴定唾液蛋白 它们调节细菌与人口腔上皮细胞的黏附 细胞。AIM II是一种免疫学、生化和 旨在系统鉴定和鉴定的微生物学研究 唾液蛋白及其多肽的定量测定 降解，为细菌提供特定的结合部位 黏附在牙齿上或以其他方式调节细菌黏附。 针对相关蛋白质和蛋白的多克隆和单抗 多肽将被制造并用于(A)开发免疫分析以 确定和测量唾液和牙齿中存在的部分 膜，(B)建立存在和 特定唾液蛋白(S)或多肽(S)的浓度和 口腔微生物的粘附性。AIM III是一种生化和 旨在(A)阐明分子基础的微生物学研究 中确定的蛋白质的黏附调节特性 目的I，通过测序，通过研究定义的分子片段 通过特定的蛋白质分解，并通过测定 对黏附调节活性的特定化学修饰， (B)调查合成多肽和天然多肽的使用情况以及 低聚糖类似物以扩展结构-活性研究，以及 (C)确定使用以下方法修改HA表面的效果 已知的HA反应蛋白的分子片段，以及 合成肽对微生物黏附的影响。来自这些的结果 多学科研究对于理解最初的 在牙菌斑的形成过程中，他们应该提供一个 为开发新的防治方法奠定了基础。